Control and Dexamethasone-treated c2c12 cells from day 3 (proliferation) have been flurocently labelled for the glucocorticoid receptor using immunocytochemistry. Describe the differences in localization of this receptor that was observed between the control and the dexmethasone treated cells.
Homework Answers
Glucocorticoid receptor mediates the cellular response to glucocorticoid. Glucocorticoid receptor is abundantly expressed C2c12 myogenic differntiation.
Subcellular localization of GR in control C2c12 is primarily in the cytoplasm. Upon dexamethason exposure , GR is activated and translocates to the nucleus in the c2c12 cells.
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Control and Dexamethasone-treated c2c12 cells from day 3 (proliferation) have been flurocently la...
What is the main take home result from Figure 1 part A? What is the question...
What is the main take home result
from Figure 1 part A?
What is the question that is being asked in figure 1 part B?
I need help with both questions please and can you explain how
you got the answers.
D Asc D As c D As GR Vinc 2 hr 4 hr 6 hr CD A/D CDA/D C D A/D GR Vinc Figure 1: Western Blot of the effect of treatments on total and cytoplasmic glucocorticoid receptor (GR)...
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I need help with a and b please.
4) Based on the image below (from Dean et al 2001), answer the following questions (7). Serine/threonine protein phosphatase 5 (PP5) Control Nuclear > Cytoplasmic Localization of GR-GFP (%) ISIS 18321 Dermethasone Control Dexamethasone Dexamethasone Figure 2: Effects of ISIS 15534 and dexamethasone on the subcellular distribution of GR-GFP. A. Representative subcellular distribution of GR-GFP. A549 cells were grown and treated with ISIS 15521 (a mismatched control antisense oligonucleotide for ISIS 1535),...
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What is the main take home result
from Figure 1 part A?
What is the question that is being asked in figure 1 part B?
I need help with both questions please and can you explain how
you got the answers.
D Asc D As c D As GR Vinc 2 hr 4 hr 6 hr CD A/D CDA/D C D A/D GR Vinc Figure 1: Western Blot of the effect of treatments on total and cytoplasmic glucocorticoid receptor (GR)...
Untreated cells Paclitaxel treated Nocodazole treated In these images, cells have been stained fluorescently for nuclei in blue, and microtubules in green From these images, please describe the effects of paclitaxel and nocodazole on microtubule structure FT O BI U Do E = g QiLeam Video
I need help with a and b please.
4) Based on the image below (from Dean et al 2001), answer the following questions (7). Serine/threonine protein phosphatase 5 (PP5) Control Nuclear > Cytoplasmic Localization of GR-GFP (%) ISIS 18321 Dermethasone Control Dexamethasone Dexamethasone Figure 2: Effects of ISIS 15534 and dexamethasone on the subcellular distribution of GR-GFP. A. Representative subcellular distribution of GR-GFP. A549 cells were grown and treated with ISIS 15521 (a mismatched control antisense oligonucleotide for ISIS 1535),...
Before control charts are invented, the process engineers have been routinely making line charts that illustrate the relationship between the quality characteristic measurements and the measurement index. These line charts show how the quality characteristics vary over time. Describe at least two differences between control charts and these line charts and discuss what are the benefit of using control charts over using line charts.
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