HomeworkLib
Question

You are trying to determine the Km for an enzyme. Due to a lab mishap, you have only two usable d...

You are trying to determine the Km for an enzyme. Due to a lab mishap, you have only two

usable data points:

Substrate concentraion (microM) Reaction Velocity (microM .s^-1)

1)1 5

2)100 50

Please help not sure what to do but I know this answer can be obtained from the Michaelis equation and a Line-Weaver Burk equation isnt needed. Thank you

science   chemistry   
1 0
Add a comment Improve this question Transcribed image text
Next > < Previous
Sort answers by oldest

Homework Answers

Answer #1

we haue man oo.kma Jenom e2.① and e?

Comment if any problem

2 0
Add a comment
Know the answer?
Add Answer to:
You are trying to determine the Km for an enzyme. Due to a lab mishap, you have only two usable d...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions

  • i need help with all question 5 please! thank you Question 5: Use the data below...

    i need help with all question 5 please! thank you Question 5: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo (UM/s) a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest [S]? c) Using your Vmax estimate, calculate ! Vmax, and using your curve, estimate Km. 1/[S] M 8 1/VO (s/UM) 3.85E-03 [S]...

  • The following data set was collected from an experiment conducted in the lab where a new...

    The following data set was collected from an experiment conducted in the lab where a new enzyme is being characterized. Use the Lineweaver-Burk method in order to determine the values of KM and Vmax. Complete your work on a separate piece of paper and upload the excel file or picture of your work. Your work must include the following: Table of reciprocals Reciprocal plot (straight-line graph) Calculations and results for KM and Vmax In order to get full credit for...

  • thank you a) Do a Michaelis-Menton plot of the enzyme assay data below. Substrate Concentration mg/mL...

    thank you a) Do a Michaelis-Menton plot of the enzyme assay data below. Substrate Concentration mg/mL Velocity umole/(min-mL) 10 15 20 31 50 75 100 125 250 330 380 437 524 612 662 678 696 713 Have the substrate concentration as the x-axis of the graph, and the velocity as the y-axis. . Use a grid line every 10 substrate concentration units, and a grid line every 20 velocity units. . In Excel, gridlines can be controlled under the Chart...

  • 2. hypothesize the best conditions (pH and temperature) under which the enzyme chymotrypsin functions with an...

    2. hypothesize the best conditions (pH and temperature) under which the enzyme chymotrypsin functions with an appropriate reference. also, hypothesize what will occur in the presence of an inhibitor and the type of inhibition. EXPERIMENT 5: ENZYME ACTIVITY WITH a-CHYMOTRYPSIN Prelab Assignment 1. Prepare a flow chart, covering one half of the experimental work (either part A and C if your lab bench is on the window side of the lab or part B and D if your locker is...

  • how do you make a lineweaver-burk plot where the trend line extends backwards? is there a...

    how do you make a lineweaver-burk plot where the trend line extends backwards? is there a way of figuring out how far back it should extend based of your data ? As well, how do you plot two sets of data on one graph ? Thank you! (idk if you need to see my data-> attached below) Biochemistry Enzyme Kinetics Assignment Four answers for this assignment will be completed in elearn: En in elearn: Enzyme Kinetics Quiz ots but must...

  • Enzyme Kinetics Lab Guide Table 2: Absorbance values per minute for varied substrate concentrations Time (min)...

    Enzyme Kinetics Lab Guide Table 2: Absorbance values per minute for varied substrate concentrations Time (min) Tube A (0mM) Tube B (2.0mM) Tube C (5.0mM) Tube D (8.0mM) Tube E (12.0mM) Tube F (20.0mM) 0 0 0.012 0.066 0.066 0.076 0.191 1 0.011 0.045 0.128 0.128 0.164 0.200 2 0.017 0.096 0.190 0.190 0.219 0.241 3 0.009 0.122 0.234 0.234 0.294 0.313 Table 3. Absorbance values versus substrate concentration at a constant time (from Table 2) Concentration (mM) 0 mM...

  • can someone help me with this physics Lab Vibrating String Experiment I'm confused please and thank...

    can someone help me with this physics Lab Vibrating String Experiment I'm confused please and thank you I have This data for the Five-time I did the Experience Vibrating String Experiment Objective To study the factors affecting the velocity of waves in a stretched string. Apparatus Linear transducer, function generator, string, meter stick, mass set, table clamp with pulley. Optional: strobe light. Part A Attach one end of a string to the motor and pass the other end of the...

  • can someone help me with this physics Lab Vibrating String Experiment I'm confused please and thank...

    can someone help me with this physics Lab Vibrating String Experiment I'm confused please and thank you I have This data for the five-time I did the Experience Vibrating String Experiment Objective To study the factors affecting the velocity of waves in a stretched string. Apparatus Linear transducer, function generator, string, meter stick, mass set, table clamp with pulley. Optional: strobe light. Part A Attach one end of a string to the motor and pass the other end of the...

  • I have to do lab report and I'm stuck with the discussion only. If someone can...

    I have to do lab report and I'm stuck with the discussion only. If someone can help to write it it will be very nice. EXPERIMENT 3 THE DETERMINATION OF HYDROGEN PEROXIDE BY IODOMETRIC TITRATION ___________________________________________________________ LEARNING AIMS To improve titration technique To determine the concentration of a hydrogen peroxide solution LEARNING OUTCOMES To evaluate the use of titrimetric analysis To manipulate common volumetric apparatus To carry out standard calculations involving concentrations and moles DIRECTED READING Vogel’s Textbook of Quantitative...

  • Based on the document below, 1. Describe the hypothesis Chaudhuri et al ids attempting to evaluate;...

    Based on the document below, 1. Describe the hypothesis Chaudhuri et al ids attempting to evaluate; in other words, what is the goal of this paper? Why is he writing it? 2. Does the data presented in the paper support the hypothesis stated in the introduction? Explain. 3.According to Chaudhuri, what is the potential role of thew alkaline phosphatase in the cleanup of industrial waste. CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP 8.5, 9, 9.5, 10,...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT