Question

OH cr nC-labeled glucose at C6 13C-labeled pyruvate at C3 a) When glucose is enriched with C at C6, it will form pyruvate enriched at C3 (above). The labeled Where does the label exist in succinyl-CoA after the 'C acetyl-CoA is processed in the first round of TCA (after a single action by the first four enzymes in TCA). Circle the atom/atoms where the label exists on the molecule below d pyruvate will be converted into 1'C acetyl-CoA and then enter TCA. b) Where does the label exist in malate (below) after the C acetyl-CoA is processed in the first round of TCA? Circle the atom/atoms where the label exists on the molecule below Where does the label exist in succinyl-CoA after a second round of TCA (one full turn of the cle plus the action of the first four enzymes a second time)? Assume the acetyl-CoA that is corporated into oxaloacetate in the second round is unlabeled.

Answer 1

" 13C ל oxalnectal eo ecH2. 20 13 но nur NADHH ista

OML 13 13 レ。、 Eutc) t3 o 0 Sc&A uCe mrt) H2 O 0 0 malala