Homework Answers
Density of protein (standard) = 1.350 g/ml
Density of biuret reagent = 1.4671g/ml
Thus, mass of protein used = 2.025 gms
Mass of biuret reagent used = 2.2005 gms
Mass fraction of protein in made up sample= 0.48
Concentration of protein in std bsa = 0.48*2 = 0.96 mg/ml
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Question 1 (1 point) The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1...
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent ...
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in the assay for a tube using 0.1 mL of the 2.0 mg/mL standard BSA. Your Answer: Answer units
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the protein concentrations of a series of pet food extract samples. The BSA Standard Curve had a slope of 0.0173 Data obtained for the pet food are shown in the table below. Tubes 1-2 contained different volumes of the pet food extract. Tube # Volume Sample (UL) A(595) Volume Volume Water Reagent (3.0 (L) mL) 803 503 1 2 20 50 0.293 0.657 What is...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
Complete the purification table below for that group using the information supplied in Tables 1 &...
Complete the purification table below for that group using the information supplied in Tables 1 & 2. You may refer to the description in your lab manual on how to calculate these parameters and account for any dilutions. Sample Activity Total (ABS/min/mL) activity (ABS/min OR U) Protein Concentration (mg/mL) Total Protein (mg) Specific activity (ABS/min/mg OR U/mg) Fold Purification RP PPE [28 marks] Table 1: Triplicate absorbance data of Group A for the catechol assay of banana PPO after 2...
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample....
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample. This method uses the same principles of the standard curve from this experiment. In a Bradford Assay, a standard curve is prepared using a known protein, typically Bovine Serum Albumin (BSA), but rather than measuring the density, we can measure a different property - its 'absorbance'. The graph below is representative of a standard curve from a Bradford Assay (with the line equation displayed...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
Both Biuret and Bradford methods was used to determine unknown concentrations of both BSA and BIg....
Both Biuret and Bradford methods was used to determine unknown concentrations of both BSA and BIg. The methods followed is described in detail in the Background section. The following results was obtained: Table 1: Absorbance at 595nm obtained for different concentrations of protein standards using the Bradford method [Protein] (mg/ml) 0.00 0.125 0.25 0.50 0.75 1.00 1.50 2.00 Unknown Abs. BSA 0 0.130 0.350 0.620 0.870 1.120 1.400 1.60 1.050 Abs. BIg 0 0.080 0.130 0.360 0.520 0.650 0.850 1.080...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
2.(1 point) You have 125 μLof a protein sample. Of this, you dilute30μLinto 120μLof buffer. 50μL...
2.(1 point) You have 125 μLof a protein sample. Of this, you dilute30μLinto 120μLof buffer. 50μL of that dilution were used for a Bradford assay and produced an absorbance of 0.53.Using the standard curveyou built in the lab using BSA, find the protein concentration (in μg/μL) of the original protein sample. Show your work. (Hint: first find the dilution fold of the sample that was used for the assay.) Note: equation from standard curve: y=0.0093x + 0.1877 show all work.
please explain in details and explanation Assume you HAVE to prepare your samples in a 1.5...
please explain in details and explanation Assume you HAVE to prepare your samples in a 1.5 mL ep tube, do you think you can make 2.0 mg BSA / 1 mL Water concentration sample in a 1.5 mL ep tube? If so, please explain to me. If not, please explain to me why.
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in the assay for a tube using 0.1 mL of the 2.0 mg/mL standard BSA. Your Answer: Answer units
The Biuret protein assay is performed by mixing 1.5 mL protein sample with 1.5 mL Biuret reagent and incubating the sample 15 min at room temp. Calculate the protein concentration in...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the protein concentrations of a series of pet food extract samples. The BSA Standard Curve had a slope of 0.0173 Data obtained for the pet food are shown in the table below. Tubes 1-2 contained different volumes of the pet food extract. Tube # Volume Sample (UL) A(595) Volume Volume Water Reagent (3.0 (L) mL) 803 503 1 2 20 50 0.293 0.657 What is...
4. (a) Determine the experimental concentration of protein in the unknown solution in mg/mL using the Part II Data Table and the equation on page 2 for the absorbance assay at 280 nm. Hint: Convert the molar concentration (M or moles/liter) of BSA from absorbance assay at 280 nm method to mg/mL, assume that the molecular weight of BSA = 66.5 kDa [7 points 6500 g/mol Post-lab 10 Report Form: Determination the Concentration of a Protein You must show your...
Complete the purification table below for that group using the information supplied in Tables 1 & 2. You may refer to the description in your lab manual on how to calculate these parameters and account for any dilutions. Sample Activity Total (ABS/min/mL) activity (ABS/min OR U) Protein Concentration (mg/mL) Total Protein (mg) Specific activity (ABS/min/mg OR U/mg) Fold Purification RP PPE [28 marks] Table 1: Triplicate absorbance data of Group A for the catechol assay of banana PPO after 2...
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample. This method uses the same principles of the standard curve from this experiment. In a Bradford Assay, a standard curve is prepared using a known protein, typically Bovine Serum Albumin (BSA), but rather than measuring the density, we can measure a different property - its 'absorbance'. The graph below is representative of a standard curve from a Bradford Assay (with the line equation displayed...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
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