In a Western Blot performed on MAP kinase, 2 weight bands showed up; one at 44 kDa and one at 48kDa . I know that there...
In a Western Blot performed on MAP kinase, 2 weight bands showed up; one at 44 kDa and one at 48kDa . I know that there should be 2 bands, but why?
Homework Answers
Given that a western blot is performed on MAP kinase and it gave
rise to 2 weight bands.
These bands correspond to ERK1 and ERK2. One of these is
phosphorylated form and is
detected by phospho ERK antibody and other is a band which is
detected by nonphosphorylated
ERK antibody. These two give rise separate bands on the blot.
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In a Western Blot performed on MAP kinase, 2 weight bands showed up; one at 44 kDa and one at 48kDa . I know that there...
Name at least two potential proteins within this pathway that could be Mutant A. Explain why. wt cells Mutant A Mutant B Mitogen 120 KDa | 110 KDa 100 KDa- 65KDa Figure 1: Newly identified MAP kinase...
Name at least two potential proteins within this pathway that
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Explain why.
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You find yourself setting up another Western blot, however. today you are preparing the samples t...
You find yourself setting up another Western blot, however. today you are preparing the samples to load onto the SDS-PAGE gel. You are using a 4-20%SDS-PAGE gel that can hold a maximum of 30 μしper 1 You remember from you so decided to prepare each sample with a final volume of 25 uL. However, no one is perfect at pipetting, so make sure each sample is made at 1.5X the total volume needed per well. The protein you are interested...
this is western blotting experiment and I need your help with questions. I will send the...
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One of AT&T's core 4G LTE bands is band 2, which operates at 1900 MHz. If...
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The one I selected is incorrect. Please let me know why and thank you. Why should...
The one I selected is incorrect. Please let me know why and
thank you.
Why should the block move at constant velocity (due to the pull of the hanging weight) when finding kinetic friction (procedure 2). Constant velocity means zero acceleration, means Tension = Friction force Because we cannot measure acceleration. if velocity is not constant, the tension in the string wil become zero. Because acceleration will add another force pulling the block (in addition to the tension in the...
I know drosophila only has recombination among females. So since 20 map units=20% recombination, I HAVE...
I know drosophila only has recombination among females. So since
20 map units=20% recombination,
I HAVE THE ANSWERS! I need help understanding
why the answers are what they are. PLEASE don't just copy
the answers I will write down
a) 1/2
b) (.1)/(.4)(.1) = 20%
c) 2:1
d)
1/3 w female
1/3 w+ female
1/15 w male
4/15 w+ male
I NEED AN EXPLANATION OF THE ANSWERS. NOT THE ANSWERS
THEMSELVES. THANK YOU IN ADVANCE.
12. A Drosophila female is...
I know how to do number one just not number 2. Please answer number 2 only...
I know how to do number one just not number 2. Please answer
number 2 only
Write a java program that will print out following pattern. Use nested for loop. 2. Add a method to the above program that generates takes two numbers as parameters and prints out all the numbers from number 1 to number 2 separated by using a while loop. (Do not print the last ,'). For example: Calling method1(9,51) should print: 9, 10, 11, 12, 13,...
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I need help with number 4 2. 3. Your Experimentally Determined Freezing Point a Point 44°C...
I need help with number 4
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Ignore the handwritten answers, but in reality I would like to know if this is a...
Ignore the handwritten answers, but in reality I would like to
know if this is a one tailed or two tailed probability and
why
um Bein Sugor BUAL 2650-Section 6 (11:00 am) In Class Exercise 2 (10 points) believed supermarket brands were as good as A study by Consumer Reports showed that 64%ofsupermarketsh pers national name brands in terms of product manufacturer of a national.oame-brand ketchup product asked 100 supermarket shoppers supermarket brand of ketchup was as good as the...
Name at least two potential proteins within this pathway that
could be Mutant A.
Explain why.
wt cells Mutant A Mutant B Mitogen 120 KDa | 110 KDa 100 KDa- 65KDa Figure 1: Newly identified MAP kinase signaling pathway mutants exhibit opposite effects. WT, Mutant A, and Mutant B cells were (+), and were not (), exposed to a signaling pathway stimulatory mitogen. Random mutations introduced to CHO cells then downstream S-cyclin expression was monitored to indicated MAP kinase signaling...
You find yourself setting up another Western blot, however. today you are preparing the samples to load onto the SDS-PAGE gel. You are using a 4-20%SDS-PAGE gel that can hold a maximum of 30 μしper 1 You remember from you so decided to prepare each sample with a final volume of 25 uL. However, no one is perfect at pipetting, so make sure each sample is made at 1.5X the total volume needed per well. The protein you are interested...
this is western blotting experiment and I need your help with
questions. I will send the summary for this exiperiment
PRE-LAB PROTOCOL: Answer the following pre-lab questions: 1. 2. 4. How is mouse anti-goat lgG antibody generated? Why must sodium azide not be included in the secondary antibody incubation steps? Assuming that the overall yield of LDH enzyme in your prep was exactly 100%, how should the intensity of the LDH band in the NADH pool sample compare to that...
One of AT&T's core 4G LTE bands is band 2, which operates at 1900 MHz. If we made a quarter wave monopole antenna designed for this band, what should the length of the antenna be? AT&T also owns band 5 (850MHz), also used for LTE. Which of these two bands (band 5 vs band 2) would provide higher data rates, and why? Comparing GHz vs Khz frequency radiation for radio communication, what is one 1 disadvantage of the higher frequency...
The one I selected is incorrect. Please let me know why and
thank you.
Why should the block move at constant velocity (due to the pull of the hanging weight) when finding kinetic friction (procedure 2). Constant velocity means zero acceleration, means Tension = Friction force Because we cannot measure acceleration. if velocity is not constant, the tension in the string wil become zero. Because acceleration will add another force pulling the block (in addition to the tension in the...
I know drosophila only has recombination among females. So since
20 map units=20% recombination,
I HAVE THE ANSWERS! I need help understanding
why the answers are what they are. PLEASE don't just copy
the answers I will write down
a) 1/2
b) (.1)/(.4)(.1) = 20%
c) 2:1
d)
1/3 w female
1/3 w+ female
1/15 w male
4/15 w+ male
I NEED AN EXPLANATION OF THE ANSWERS. NOT THE ANSWERS
THEMSELVES. THANK YOU IN ADVANCE.
12. A Drosophila female is...
I know how to do number one just not number 2. Please answer
number 2 only
Write a java program that will print out following pattern. Use nested for loop. 2. Add a method to the above program that generates takes two numbers as parameters and prints out all the numbers from number 1 to number 2 separated by using a while loop. (Do not print the last ,'). For example: Calling method1(9,51) should print: 9, 10, 11, 12, 13,...
I need help with number 4
2. 3. Your Experimentally Determined Freezing Point a Point 44°C 17 Use the reference value you looked up for the melting point of Phenyl Salicylate Value for Freezing Point 47-43°C 144-41100 7.32% B. Calculate the Percent Error 41 X 100 Charles' Law and the Determination of Absolute Zero Graph Temperature vs. Length of Gas Column. Temperature should be on the "X axis and Length on the TV axis. The range on the yaxis must...
Ignore the handwritten answers, but in reality I would like to
know if this is a one tailed or two tailed probability and
why
um Bein Sugor BUAL 2650-Section 6 (11:00 am) In Class Exercise 2 (10 points) believed supermarket brands were as good as A study by Consumer Reports showed that 64%ofsupermarketsh pers national name brands in terms of product manufacturer of a national.oame-brand ketchup product asked 100 supermarket shoppers supermarket brand of ketchup was as good as the...
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