Question

What number spikes on a chromatogram? I am trying to draw a chromatogram of a partial genetic fingerprint. For example TPOX allele 1 with a repeat structure of [GAAT]5 length of flanking 120 and total length of fragment is 150 (bp). What number spikes on the chromatogram or what would this look like on a chromatogram?

Answer 1

In a chromatogram spike is showing any artifact or it means either expired polymer, or presence of air bubbles which used to obtained in the polymer block tubing. some other reason for spike in chromatogram could Possibly be contaminant or crystal deposits in the polymer

chromatogram of TPOX allele 1 with a repeat structure of [GAAT]5 length of flanking 120 and total length of fragment is 150 (bp)

90 120 150 180 210 240 270 300 330 360 390 420 TPOX 3000 TH01 2500 CSF D7 D16 D3 DE 2000 D13 D21 D8 Penta D D18 FGA VWA 1500 AMEL Penta E 1000 500 TPOX allele 1 with a repeat structure of [GAAT]5 length of flanking 120 and total length of fragment is 150 (bp)