1. It will lose the cofactors that are required for full activity.
2. The protein will not be able to maintain the conformation in dilute solutions without the proper osmoticum.
why do we start with the highest dilution (lowest amount of enzyme) when running the enzyme...
When running enzyme assays, why do you need to normalize enzyme activity to total protein concentration? What does the specific activity mean in the research lab where enzymes are used? (Hint: are enzymes always pure?)
If we start with an original tube of bacteria and tube 1 is a 1:10 dilution of the original, tube 2 is a 1:10 dilution of tube 1, and tube 3 is a 1:10 dilution of tube 2, what is the total dilution of tube 3? Show your calculations.
General Biology / Biol 101 REVIEW QUESTIONS ON ENZYME KINETICS 10) Why did we do all of our work in a water bath and use buffer in all of our tubes? 11) What happens to velocity of an enzyme catalyzed reaction as we increase the amount of initial substrate available with a constant amount of enzyme? Be specific. 12) What is the purpose of calculating Km? (What does it tellus about an enzyme?) 13) What are the advantages of the...
why you will start by filtering the 1 ml dilution of river water and working your way up to the 50 ml dilution and not the other way around (in membrane filter technique)
why do we not perform a 1/10^6 dilution (or even 1/10^3) directly straight from the stock solution?
Q: Arrange according to booling point from highest to lowest and explain why ? Aniline , N,N dimethylaniline , N,ethylaniline .... Thanks :)
When and why do we do a nursing reassessment
why do we add an when naming organic compounds. example is why do we write methanal and not methal
Mayo is among the lowest cost and highest quality health care systems in the US. How do we get the rest of the country to follow suit or shouldn't we?
Vmax of an enzyme-catalyzed reaction is A. the rate observed when the enzyme active sites are saturated with substrate B. independent of the amount of enzyme present C. the rate observed at the highest substrate concentration that can be experimentally obtained D. the initial rate observed at very low substrate concentrations