why do we not perform a 1/10^6 dilution (or even 1/10^3) directly straight from the stock...
why do we not perform a 1/10^6 dilution (or even 1/10^3) directly straight from the stock solution?
Homework Answers
We do not perform such a high dilution from a highly concentrated stock solution as this would involve manual pipetting error. Moreover, serial dilution of a concentrated stock solution is always the better way to do it.
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why do we not perform a 1/10^6 dilution (or even 1/10^3)
directly straight from the stock...
Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic...
Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic solution of 100 mg/mL, what volume of this stock should you add to 10 mL sterile broth to obtain a final concentration of 64 micrograms/mL? mL
Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic...
Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic solution of 100 mg/mL, what volume of this stock should you add to 10 mL sterile broth to obtain a final concentration of 64 micrograms/mL? mL
"Prepare 500 mL of a 0.1 M sodium hydroxide solution by dilution of a 1 M...
"Prepare 500 mL of a 0.1 M sodium hydroxide solution by dilution of a 1 M sodium hydroxide stock reagent or from solid NaOH. This dilution need only be performed to 5% accuracy, that is, using a graduated cylinder and an Erlenmeyer flask." 1. Explain why it is not necessary and even not desirable to perform the preparation of 1 M sodium hydroxide operation with higher accuracy. "Add the analyte to the beaker and fill with distilled deionized water to...
Describe how to do a serial dilution of a 1 x 10-3 M solution to obtain...
Describe how to do a serial dilution of a 1 x 10-3 M solution to obtain 1 L of a 1 x 10-9 M solution. Please include necessary volumes and equipment that would need to be used in a lab.
You need to perform a 5 x 10-6 dilution. Give a detailed scheme of how you...
You need to perform a 5 x 10-6 dilution. Give a detailed scheme of how you could carry out this dilution using serial dilutions similar to those you performed in class.
If we start with an original tube of bacteria and tube 1 is a 1:10 dilution...
If we start with an original tube of bacteria and tube 1 is a 1:10 dilution of the original, tube 2 is a 1:10 dilution of tube 1, and tube 3 is a 1:10 dilution of tube 2, what is the total dilution of tube 3? Show your calculations.
Answer 3) The concentration of phosphorous in a stock solution is 10.0 ppm. After the dilution...
Answer
3) The concentration of phosphorous in a stock solution is 10.0 ppm. After the dilution by DI water, three new solutions are prepared as shown in the following table. Find the concentration of phosphorous in these solutions. Show all calculation processes to receive the credit. (6 pts) Phosphorous Concentration After dilution (ppm) Solution # Stock Solution (mL) Total Volume After dilution (ml.) 25.00 2.50 1 50.00 2 8.00 250.0 3 50.00
why do we start with the highest dilution (lowest amount of enzyme) when running the enzyme...
why do we start with the highest dilution (lowest amount of enzyme) when running the enzyme reactions
3. Why can we not measure the rate of glucose formation by cellobiase directly? (2 points)...
3. Why can we not measure the rate of glucose formation by cellobiase directly? (2 points) 4. How do we use analogs of cellobiose to measure the reaction rate of cellobiase activity? Which analog of cellobiose did we use in the lab today? (4 points) 5. Why do we need to use a stop solution in our experiments today? (2 points
Serial Dilution Questions 1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution...
Serial Dilution Questions
1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2,00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of...
Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic solution of 100 mg/mL, what volume of this stock should you add to 10 mL sterile broth to obtain a final concentration of 64 micrograms/mL? mL
Lab Exercise 13: 1. Perform the following dilution calculation. If you have a sterile stock antibiotic solution of 100 mg/mL, what volume of this stock should you add to 10 mL sterile broth to obtain a final concentration of 64 micrograms/mL? mL
Answer
3) The concentration of phosphorous in a stock solution is 10.0 ppm. After the dilution by DI water, three new solutions are prepared as shown in the following table. Find the concentration of phosphorous in these solutions. Show all calculation processes to receive the credit. (6 pts) Phosphorous Concentration After dilution (ppm) Solution # Stock Solution (mL) Total Volume After dilution (ml.) 25.00 2.50 1 50.00 2 8.00 250.0 3 50.00
Serial Dilution Questions
1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2,00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of...
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