Question

1. What is the concentration of this solution?

The solution consisted of 1 mL of BA and 50 μL of Trypsin.

BA consisted of 0.526 mM Na –benzoyl-D,L,-arginine p-nitroaniline hydrochloride in TRIS butter.

Trypsin (X) solution consisted of 2.00 mg/mL Trypsin in TRIS buffer

Answer 1

So as per the equation of volumerty, Molarityx Volume is a contant or MV= constant.

we have 2 solutitions mixed to form one solution. so the above equation will be transformed into

M1V1+M2V2= M3 V3

we want to find M3

Fro the question it is given

M1=0.526 mM

V1=1mL

V2=50 microL

M2 is not given but can be calculated from the m/v ratio. 2mg/ml

V3 will be 1 ml+50 uL =1.05 ml

M2 =2 x 1000/Mw- where Mw is the molecular weight of trypsin,=23300 Da (da almost similar to amu)

M2=0.086M

So M3 =(0.0526 mM* 1 mL +0.086M * 50uL ) 1.05mL

M3 =(0.0526 mM* 1 mL +0.086mM * 50mL ) 1.05mL = (since ul =1/1000ml ( power will be same on multiplication)

M3=4.15mM