Homework Answers
ANS1 - 2
ANS46 - Cas9 loads with a sgRNA sequence complementary to about 20 nucleotide sin the DNA target sequence,and this is basically where Cas9 will cut the DNA.
ANS39 - DNA with more of a negative charge is drawn more quickly towards the anode(the positively charged electrode).
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The picture above represents an agarose gel that was used to analyze plasmid DNA after it...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this...
1. A circular plasmid has two PmeI restriction sites. A PmeI restriction enzyme will cut this plasmid into two fragments. A. True B. False 2.In general, restriction enzymes that recognize four nucleotides have higher probability to produce more DNA fragments than those enzymes that recognize six nucleotides. A. true B. false 3. Which of the following sequences are palindromes? A. 5' TGGCCA 3' B. 5' GAAAAG 3' C. 5' CGATGG 3' D. 5' GACGAC 3' 4. Below are the possible...
RESTRICTION DIGEST ANALYSIS QUESTIONS(true or yes = A: false or no = b) 1. Larger DNA...
RESTRICTION DIGEST ANALYSIS QUESTIONS(true or yes = A: false or no = b) 1. Larger DNA fragments appear near the bottom of the gel. 2. Larger DNA fragments move more rapidly through the gel. D ONA that has many restriction sites for a certain endonuclease will be cut into more fragmets than DNA with fewer restriction sites. 4. Cutting DNA with many different endonucleases will result in more DNA fragments. 5. Restriction enzymes all recognize the same base sequence when...
Write true or false ______ 1. The DNA sequence of one human being is on average...
Write true or false ______ 1. The DNA sequence of one human being is on average 99.9% identical to another random human being. ______ 2. As of 2009, all living human beings have had their entire genome sequenced. ______ 3. The nucleotide bases present in a DNA sequence are A, U, G, C. ______ 4. Techniques that enabled scientists to clone genes were developed in the 1970s. ______ 5. A restriction enzyme is useful because it is a generic enzyme...
Sketch the predicted migration pattern on an agarose gel of the following DNA samples: (1) Undigested...
Sketch the predicted migration pattern on an agarose gel of the following DNA samples: (1) Undigested pET15b plasmid; (2) pET15b digested with the EagI restriction enzyme; (3) pET15b digested with NdeI and AhdI (see pET15b vector map for location of these enzyme cutting sites). Include in your sketch a DNA base-pair ladder from 0 to 10,000 bp with 1000 bp increments. Label each band in the DNA ladder with the known size (in base-pairs), and estimate the size of the...
can someone explain throughly on how to find a-c??? thanks!!! The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel be...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
question number 7 purifying plasmid from your cells, you will set up a teaction to cut...
question number 7
purifying plasmid from your cells, you will set up a teaction to cut the plasmid DNA with the restriction enzyme Bgl Il. See the map below for the positions of the Bgl Il restriction enzyme sites on the plasmid. How many DNA fragments would you expect if your restriction enzyme digest was complete and your plasmid contains insert? Question 7 Not yet graded/ 1 pts After you electrophoresed the plasmid digest in Question 6, your gel showed...
Which of the following is the correct order of events in the polymerase chain reaction (PCR)?...
Which of the following is the correct order of events in the polymerase chain reaction (PCR)? See Section 20.2 (Page 468) cooling to allow primers to attach; heating to separate double-stranded DNA; elongation of DNA strand as nucleotides are added elongation of the DNA strand as nucleotides are added; cooling to allow primers to attach; heating to separate double-stranded DNA heating to separate double-stranded DNA; cooling to allow primers to attach; elongation of DNA strand as nucleotides are added heating...
2. Here are some DNA fragments that have been isolated by gel electrophoresis after being cut...
2. Here are some DNA fragments that have been isolated by gel electrophoresis after being cut with restriction enzynes. A. 5 '-ACTGACATAGGCACCCCTTAA-3 3'-TGACTGTATCCGTGGGG-5 5 '-TGACTGTATCCGTGGGG-3' 3 '-ACTGACATAGGCACCCCTTAA-5' 5 '-GGCATACTAGATCCACGTTAA-3 3'-CCGTATGATCTAGGTGC-5 5 '-GGCATACTAGATCCACGAATT-3 3'-CCGTATGATCTAGGTGC-5 E. 5 '-GGCATACTAGATCCACGGATC-3 3'-CCGTATGATCTAGGTGC-5 a. Which pair of these fragments has appropriate complementary sticky ends to get joined together in a recombinant DNA molecule? b. What enzyme would we use to join up the DNA backbones to make the make the recombinant molecule?
7.. In the controlled termination method of DNA sequencing, reading the gel from _____ gives the...
7.. In the controlled termination method of DNA sequencing, reading the gel from _____ gives the sequence in the _____ direction; _____ fragments that were terminated _____ in polymerization move faster down the gel a. bottom to top; 5′ to 3′; shorter; early b. top to bottom; 5′ to 3′; longer; early c. bottom to top; 5′ to 3′; longer; later d. top to bottom; 5′ to 3′; shorter; early e. bottom to top; 3′ to 5′; shorter; early 8....
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
RESTRICTION DIGEST ANALYSIS QUESTIONS(true or yes = A: false or no = b) 1. Larger DNA fragments appear near the bottom of the gel. 2. Larger DNA fragments move more rapidly through the gel. D ONA that has many restriction sites for a certain endonuclease will be cut into more fragmets than DNA with fewer restriction sites. 4. Cutting DNA with many different endonucleases will result in more DNA fragments. 5. Restriction enzymes all recognize the same base sequence when...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
question number 7
purifying plasmid from your cells, you will set up a teaction to cut the plasmid DNA with the restriction enzyme Bgl Il. See the map below for the positions of the Bgl Il restriction enzyme sites on the plasmid. How many DNA fragments would you expect if your restriction enzyme digest was complete and your plasmid contains insert? Question 7 Not yet graded/ 1 pts After you electrophoresed the plasmid digest in Question 6, your gel showed...
2. Here are some DNA fragments that have been isolated by gel electrophoresis after being cut with restriction enzynes. A. 5 '-ACTGACATAGGCACCCCTTAA-3 3'-TGACTGTATCCGTGGGG-5 5 '-TGACTGTATCCGTGGGG-3' 3 '-ACTGACATAGGCACCCCTTAA-5' 5 '-GGCATACTAGATCCACGTTAA-3 3'-CCGTATGATCTAGGTGC-5 5 '-GGCATACTAGATCCACGAATT-3 3'-CCGTATGATCTAGGTGC-5 E. 5 '-GGCATACTAGATCCACGGATC-3 3'-CCGTATGATCTAGGTGC-5 a. Which pair of these fragments has appropriate complementary sticky ends to get joined together in a recombinant DNA molecule? b. What enzyme would we use to join up the DNA backbones to make the make the recombinant molecule?
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