Question

Refer to the diagram indicating the metabolism of lysine. It shows the conversion of L-aspartate, through a variety of intermediaries into L-lysine. For each reaction step, the enzyme that catalyzes that reaction is included on the right of the arrow. A L-aspartate L-aspartate semialdehyde pyruvate H,0 DHDPS HTPA NAD(P)H NAD(P)* DHDPR THDP meso-diaminopimelate H DAPDC CO * L-lysine

Answer 1

Based on the data provided in the experiment, enzyme mutated in each cell line are written below

Mutant K1 - DADPÇ

Only MM+ lys , cause growth of mutant K1 , means DADPC is defective, only end product is able to produce growth

And rest other media is not able to provide growth

Mutant K2 -- DHDP5

All three media provide growth of cell , means mutant is DHDP5

Mutant K3 - DHDPR