Question

5. In an equilibrium denaturation experiment, one makes up a series of different protein solutions, 1, 2, ..., M, having different amounts of urea, a denaturing agent, x1, x2, ..., XM. Then, for each particular solution, having denaturing agent in amount x, one measures the proportion f(x) of native protein molecules and the proportion fo(x) = 1 - fr of denatured protein molecules. Increasing the denaturing agent increases the population of Drelative to N. In the absence of denaturing agent, the protein is fully native. In high

Answer 1

Answer: Page 1 * According to data- =) protein folding and unfolding are two Process which are which are cooperative. It means when the protein get unstable due to high temperature, change in PH or other conditions, then initially only part of the Protein become unstable and because this part is interacting with other part of the protein, other part also stast denaturing. >> so, protein denaturation start with a small part of the protein but as the temperature increases, the part of denaturation increases and after some time reached the saturation point. This denaturation is shown in sigmoidal cuore. Х