Question

a. If you counted all of the cells in a single grid of a hemocytometer (in all nine 1mm²-squares), and you obtain a total of 189 cells, what would be the concentration of cells in your sample? Please enter your answer as "Number of cells/mL" using the scientific notation.
Number of cells/mL:

b. What would be the total number of cells if your sample volume was 5.5 mL? Please enter your answer in scientific notation.
Total number of cells:

c. What volume (in μL) of your cell mixture would you add to a 100 mm dish to get 2.55E+03 cells/cm²?
Volume:

Answer 1

to calculate number of viable cells i n hemocy to meter. 1 Calculate average count from each of sets of 16 commer squares. 2) Multiply by 10-4 (10,000) 3 multiply by 5 to correct for 1:5 dilutions by b) If total no. of viable cells – 189 cells. 189x10-4x5 – cells me. 189x5x104 cells/ml 94.5Xlo 3 cells/ml appox 100 x 102 I celes/m) to tont → toxtoto Inlml = 94.5x103 cells present quantity of sample increases to 5.5 5.5m1 94.5x10 3 x 5.5 cell (m) = 519.15X10 -3 cells/m/ 1 e