Question

The Notch gene, involved in Drosophila development, is contained within a restriction fragment of Drosophila genomic DNA produced by cleavage with the enzyme SalI. The restriction map of this Drosophila fragment for several enzymes (Sall, PstI, and Xhol) is shown here; numbers indicate the distances between adjacent restriction sites. This fragment is cloned by sticky-end ligation into the single Sall site of a bacterial plasmid vector that is 5.2 kb long. The plasmid vector has no restriction sites for PstI or Xhol enzymes. 3.0 2.0 4.0 0.2 3.3 2.5 kb P Pstl; S Sall; X Xhol Make a sketch of the expected patterns seen after agarose gel electrophoresis and staining of a Sall digest (alone), of a PstI digest (alone), of a Xhol digest (alone), of the plasmid containing vector and . Drosophila fragment. Indicate the fragment sizes in kilobases

A genetics problem covering chapter 10 concepts. Could really use some help!

Answer 1

Please look in the image for solution.

251 S 5-2) VCctor. micl est b There are two bands しis digest uuth sael. Becaus e . There are evdy tND restriction o band or Saoi in micl there are three evestrichom 2 lcb As there, are two restriculom «it pLasic. Thats whuy two proclucecl and their0z0 Marker Saul Pstl Xhol 16- 12.5 EXCELLENT