For stable integration of a fluorescent protein into the genome of a cell, what is the most useful
technique?
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Transfection |
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Viral infection |
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Electroporation |
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Pinocytosis of plasmids |
For stable integration of a fluorescent protein into the genome of a cell, what is the...
4. What is the difference between a fluorescent-tagged protein, fluorescent-tagged antibody, and a fluorescent dye? What is one advantage and one disadvantage of each? (6 marks)
which of the following answers are inccorect and what is the correct answer For the next 17 questions select one of the following virus structures to correctly complete each of the statements below Question Selected Match Confers greater susceptibility to disinfectants B. The viral envelope Is a flexible membrane composed of protein and lipid B. The viral envelope Must enter the host cytoplasm for replication to occur A. The viral genome Is affected by base analogs in viral inhibition A....
what is the viral genome, Target cells,course of infection and Prognosis of HIV?
You prepared a translational fusion of protein Z and the yellow fluorescent protein YFP (ZYFP) then expressed it in an animal cell line. You hypothesize that protein Z is a component of two different complexes in a cell. To test this idea, you intend to image Z-YFP using a fluorescence microscope equipped with a 60x objective (NA=0.9), and a 540 nm filter to detect YFP emission. Can you distinguish these complexes if they are approximately 330 nm apart? Explain why...
11. A rare autosomal dominant condition affects the following family pedigree. What is the probability that the unborn child marked with the question mark is affected by the condition? Express the answer as a decimal fraction with two digits after the decimal point. Do not round the fraction. (.e 1/3 = 0.27) 12. Which of the following is not a necessary step in lytic bacteriophage infection cycle? Select one: a. Injection of the viral genome b. Viral protein is synthesized by the host cell c. The...
You are employed at the clinic and there is a potential viral outbreak in the town. The scientists figured out a specific viral protein that can be a potential target to screen for the presence of the infection. Suddenly, you have around 500 people at your clinic who are required to be checked for the viral infection within a couple of hours. Answer the following questions: What will be the most efficient and fast technique to screen the patient for...
1. What is an inherent problem with stable transfection that does not occur during transient transfection? A Too much of the target protein may be expressed B Expression of target protein may affect additional pathways C Insertion of the construct may disrupt an endogenous gene D The target protein may be more sensitive to proteolysis 2. What is NOT one of the useful features of adding a myc or FLAG tag to a protein in a construct? A You can...
You are assigned to purify a protein from an E.coli cell lysate. The following information is provided to you about the target protein. The protein has an N-terminal 6X histidine tag (His-tag) attached to it The protein is very stable in PBS (Phosphate Buffered Saline) buffer that you need to use as the storage buffer after purification It is an enzyme that will be used as a potential drug. You have the amino acid sequence of the protein. Based on...
1. Viral integration into the cellular genome may convert a proto-oncogene into a transforming oncogene 2. Immunosuppressive drugs used to prevent graft rejection include drugs (like Imuran) that result in generalized suppression of all dividing cells and drugs that target T cells (like cyclosporine A) 3. IFNα has a direct antitumor effect, while TNFα has an indirect cytotoxic effect on tumor cells 4.Oncogenes are unique genes of a normal cell that only function to promote cell transformation. 5. Attributes of...
The exogenous DNA used in bacterial transformation can be, RONA mRNA molecule engineered plasmid red fluorescent protein Incorrect Question 4 0/0.5 pts Bacteria that did not receive a plasmid are put on an LB plate that DOES contain ampicillin. What do you expect to happen? the bacteria will create a lawn the bacteria will not grow a few colonies will be seen http:/misac.instructure.com couro/87588/quizzes/163615 7/29/2020 Review Que: BIOL-8-05-12561.202010 Transformation efficiency. Concentration of plasmid DNA. Question 8 0.5/0.5 pts Super coiled...