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Mention and make a diagram of 3 molecular mechanisms that have had a biotechnological impact in...

Mention and make a diagram of 3 molecular mechanisms that have had a biotechnological impact in recent years.

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B-galactosidase gene B-galactosidase 1Insert genes for each gene Promoter Promoter of the two insulin polyeptides next to a highly expressed gene Insulin gene for ß-galactosidase Insulin gene A subunit B subunit Antibiotic resistance gene Antibiotic resistance gene Transform E. coli with the recombinant expression vectors and select transgenic cells by their antibiotic resistance B-galactosidase/insulin A fusion protein B-galactosidase/insulin B fusion protein Purify the fusion proteins Chemically remove insulin polypeptides from the p-galactosidase protein DisulfideB bridge Active insulin Combine the polypeptides to produce functional insulin. 13.12: David Mclntyre.

In this answer, I have explained about insulin. How insulin is made by recombinant DNA technology.

A gene of insulin is taken from the organism then cloned into bacteria and then transformed cells are grown and insulin is harvested from them.

Next I explained how a vaccine is made.

The process is the same for everything that is RDT. You isolate the gene and then clone into bacterial and then isolate. We can make everything by RDT. We can make a genetically modified organism, vaccine, etc.

Next, I explained gene therapy. Gene therapy is of two types.

Ex vivo and In vivo.

If the disease is cured inside the body then it is in vivo.

If the disease is cured outside the body then it becomes ex vivo.

The last one is CRISPR. It is extra. Crispr is used for gene editing.

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