During DNA replication, both strands of DNA are separated by the enzyme helicase and replication fork is formed which moves in the direction of unwounding DNA. Short RNA primer is then synthesized by DNA primase at the replication fork which is then extended by DNA polymerase. DNA polymerases are enzymes that can replicate DNA in 5' to 3' direction only. Therefore, the template strand with 3'-5' polarity is called the leading strand as DNA polymerize can continuously replicated DNA from start of replication. However, the template strand with 5'-3' polarity is discontinuously replicated in series of short okazaki fragments by DNA polymerase because it is copied in the direction opposite to the replication fork. Therefore, due to the inherent opposite polarity of the two strands of DNA and DNA replication in only 5'-3' polarity results in a newly synthesized DNA which the result of both continuous and discontinuous DNA synthesis.
explain why any newly synthesized strand of DNA is the result of bith continuous and discontinuous...
What characteristics of DNA result in the requirement that some DNA synthesis has to be discontinuous? (Why is there a “lagging strand?”)
DNA mismatch repair enzymes preferentially repair bases on the newly synthesized DNA strand, using the old DNA strand as the template. If mismatches were simply repaired without regard for which strand served as template, would this reduce replication errors? Explain your answer (5 pts) Describe DNA replication (remember to mention all the enzymes involved) (10 pts)
in the figure below, the dashed line represents a newly synthesized DNA strand while the solidines represent parental/template DNA strands. The polarity for one parental strand is given the direction of the replication fork is indicated with an arrow. With respect to this replication fork, in which direction would helicase be working? A B C D Direction of replication
When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides are being added to the_of the growing strand. A 5 phosphate group B. A nitrogenous base C. 3' hydroxyl of deoxyribose D. 2 H of deoxyribose
Can someone draw a picture explaining why the lagging strand in the DNA replication is discontinuous?
21. In DNA replication, new nucleotides are added a. To the 5' end of each nascent strand b. To the 3'end of each nascent strand To both ends of each nascent strand d. To the 5' end of the continuous strand and the 3' end of the discontinuous strand e. To the end of the continuous strand and the end of discontinuous strand fragments 22. DNA unwinding is done by a. Ligase b. Helicase c. Topoisomerase dHexonuclease 23. Proofreading of...
To incorporate radiolabeled nucleotides into newly synthesized DNA, researchers use α-phosphorus32-labeled nucleotides, in a DNA synthesis reaction, where the α denotes the position of the radioactive phosphate moiety. Explain why the α position--rather than the β or γ positions--is the best position for the radioactive group in these experiments.
1. Why DNA replication happens in continuous and discontinuous manner? 2. Write and explain about degenerate and nested primers. 3. What are the limitations in choosing a plant species for your cloning experiment? 4. Write briefly about glyceraldehyde-3-phosphate dehydrogenase (GAPDH) enzyme and origin of GAPDG gene.
The enzymes that initiates synthesis of a new DNA strand is called _________. The name given to the short segments of RNA that is added at the beginning of all newly synthesized DNA strands is called _________.
A. Give the nucleotide sequence of the newly synthesized DNA
strands
B. Identify the leading and lagging strand
ehotooeeAAAAY AnoChronAAA?