Question

. Using the figure of the gel below, draw in the DNA bands in the left lane that would appear from the DNA of the person described in the question above. The right lane contains a molecular weight standard.

the questions are

When circular DNA is sequenced, the nucleotide base pairs are numbered starting from an fixed position on the DNA, all the way around, usually in a clockwise manner.   a DNA molecule that is 3133 base pairs long is digested with RsaI restriction enzyme recognition sites at base numbers 366, 1534, and 2207. What are the sizes of the DNA fragments that will be produced after the DNA is digested with RsaI?

When linear DNA is sequenced, the nucleotide base pairs are numbered from the start to finish.   a DNA molecule that is 3133 base pairs long is digested with RsaI restriction enzyme recognition sites at base numbers 366, 1534, and 2207. What are the sizes of the DNA fragments that will be produced after the DNA is digested with RsaI?

5000 1:00 200 1:00 1000 750 500 250

Answer 1

In case of a circular plasmid of 3133 bp, the restriction digestion by RsaI at 366 and 1534 and 2207 bp would result in the generation of 3 fragments as:

1 bp 366 bp 2205 bp 1566 bp 1534 bp

The length of 1 fragment will be: 1534 - 365 = 1169 bp

The length of 2nd fragment will be 2205 - 1534 = 671 bp

the length of 3rd fragment will be 3133- 2205 + 365 = 928 + 365 = 1293 bp

In case of a circular plasmid of 3133 bp, the restriction digestion by RsaI at 366 and 1534 and 2207 bp would result in the generation of 4 fragments as:

366 bp 1534 bp 2205 bp

The length of one fragment = 365 bp

The length of 2nd fragment = 1534 - 365 = 1169 bp

The length of 3rd fragment = 2205 - 1534 = 671 bp

The length of 4th fragment = 3133 - 2205 =928

For Circular DNA

3000 2500 2000 וווווווו 1500 1293 1169 1000 || | 750 671 500 250

For Linear DNA

3000 2500 2000 1500 ווווו0II 1169 1000 928 || | | 750 671 500 365 250

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