Question

1.When cloning a PCR product into a plasmid using restriction enzymes, the restriction enzyme recognition sequences in the PCR product most likely came from _______, and the restriction enzyme recognition sequences in the plasmid most likely came from ________.
a. A multiple cloning site / the primers
b. The primers / a multiple cloning site
c. Both came from primers
d. Both came from the multiple cloning site
e. Naturally present in the gene of interest / the multiple cloning site

i think answer is either b or c?

2.The ______________ process is triggered by abnormal or missing bases in DNA. Enzymes such as AP endonuclease remove the sugar at that base, and then DNA polymerase fills the gap.
a. Mismatch repair
b. Double strand break repair
c. Homologous recombinant repair
d. Base excision repair
e. Nucleotide excision repair

3.One way to prevent a plasmid vector that has sticky ends from re-closing without an insert (while still allowing it to close with an insert) is to:

a. Cur with a restriction enzyme that produces blunt ends.

b. Don't add ligase to the reaction

c. Cut both the vector and the insert with two different restriction enzymes.

d. Don't cut the vector.

e. Don't cut the insert.