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1) What does beta lactamase do, and how is that helpful in selecting bacteria that contain your plasmid of interest (in this

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1. Beta lactamase is an enzyme that inactivates beta lactman by the process of hydrolysis. It breaks down the peptide bond and hence is responsible for breaking the beta lactman ring making it ineffective as an antibiotic.

This can be helpful in identifying the bacteria as the bacteria that can sectrete beta lactamase are able to resist an antibiotic attack.

Hence the bacteria secreting beta lactamase can survive in the presence of antibiotics.

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