Homework Answers
Dna A protein (have ATPase activity) bind to origin of replication and melt it.
Dna B helicase (hexameric; a type of topoisomerase II) helicase unwinds the helix while proceeding in 5’?3’ direction along the lagging strand.
DNA gyrase (type II topoisomerase) removes the torsional strain produced during helix unwinding.
DNA primase or simply primase, (a DNA dependent RNA polymerase) bind to both single strands and synthesizes a short length RNA oligonucleotide (RNA primer) onto each strand in 5’?3’ direction.
DNA polymerase III: Synthesizes most of DNA during replication.
DNA Pol I: Involved in nick translation.
DNA ligase: Joins two adjacent DNA fragments on lagging strand.
RNaseH: Removes RNA primer.
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DNA Replication. Sketch a replication fork of bacterial DNA in which one strand is being replicated...
Vocabulary: DNA Replication
Vocabulary: DNA Replication A. Helicase B. Primase C. Single Strand Binding Protein (SSB) D. Topoisomerase E. Origin of Replication F. DNA Polymerase G. Leading Strand H. Lagging strand I. DNA Ligase J. Okazaki Fragment K. Replication Fork L. RNA Primer M. Topoisomerase .1. Site where the replication of a DNA molecule begins. 2. The new continuous complementary DNA strand synthesized in the direction for the replication fork. 3. A discontinuously synthesized DNA strand that elongates in a direction away from the replication fork 4. Relaxes...
Match the following (Total 24 pts) Enzyme responsible for joining DNA strands together The nascent DNA...
Match the following (Total 24 pts) Enzyme responsible for joining DNA strands together The nascent DNA strand that is being synthesized in the same direction as the replication fork. Enz 39 DNA polymerase 40 DNA helicase responsible for transcribing RNA 41DNA sliding clamp 42 Single Stranded Binding Proteins DShort, newly synthesized DNA fragments 43 RNA primer 44 DNA ligase formed on the lagging template strand E Abundle or proteins that assist in the rate of transcription of DNA to mRNA....
If the sequence of the 5'-3'DNA strand is AATGCTAC, then the complementary DNA sequence has the...
If the sequence of the 5'-3'DNA strand is AATGCTAC, then the complementary DNA sequence has the following sequence o 3-AATGCTAC-5' 3'-CATCGTAA-5 3-GTAGCATT-5 3-TTACGATG-5 Question 2 20 pts Which of the following does the enzyme primase make? phosphodiester linkages (bonds) Okazaki fragments RNA primer DNA primer In which direction does DNA replication take place? 3'to 5 5'to 5 5'to 3 3'to 3 Question 4 20 pts Which enzyme unwinds the double-stranded helical DNA starting at the origin of replication? ligase primase...
BioLoG 11. chose the order below that most closely represents the order in which the following...
BioLoG 11. chose the order below that most closely represents the order in which the following proteins participate in DNA replication. a. helicase, single stranded binging protein, primase DNA polymerase b. single -stranded binding protein, primase DNA polymerase helicase c. primase, DNA polymerase, single- stranded binding protein, helicase d. helicase, single- stranded binding protein DNA polymerase, primase. 12. what is the function of the enzyme primase during DNA replication? a. to unwind the double helix to prime it for replication...
Formation of a replication fork results in: A. continuous synthesis of DNA on the lagging strand....
Formation of a replication fork results in: A. continuous synthesis of DNA on the lagging strand. B. supercoiling in the parental DNA ahead of the fork. C. of new 3' -OH regions on the template DNA. D. binding of SSB protein on the double-stranded parental DNA. E. All of the above occur when the replication fork is formed.
If the DNA polymerase builds both chains from the same fork, how is the second strand...
If the DNA polymerase builds both chains from the same fork, how is the second strand built if DNA polymerase only synthesizes DNA one direction (S' to 3)? The lagging strand is synthesized continuously using many short fragments, called Okazaki fragments. The leading strand is synthesized discontinuously using many short fragments, called Okazaki fragments. The lagging strand is synthesized discontinuously using many short fragments, called Okazaki fragments. The leading strand is synthesized discontinuously using many short fragments, called Okazaki fragments....
D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes...
D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes the new strand of RNA primase Choose synthesizes the new strand of DNA synthesizes a short fragment of complementary RNA attach to template DNA strands to prevent hydrogen bonding Uncoils the supercoil of prokaryotic chromosomes connects Okazaki fragments of new lagging strand of DNA unwinds and unzips the double stranded DNA Single stranded binding proteins (SSB's) DNA Ligase [Choose] Helicase [Choose) DNA Gyrase (Choose]...
2. Draw replication fork, and place and label the following -DNA being copied (template)-new D -...
2. Draw replication fork, and place and label the following -DNA being copied (template)-new D - Correct 5' and 3' markings NA-helicase -primaseleading strand and Okazaki fragments
Question 25 4 pts Which of the following terms are associated with the discontinuously replicated strand...
Question 25 4 pts Which of the following terms are associated with the discontinuously replicated strand during DNA replication? I. DNA ligase II. Okazaki fragments III. lagging strand IV. leading strand V. RNA polymerase O I, II, III O I, II, III, V O II, IV, V O I, II, IV, V O II, III, V 4 pts Question 26 4 pts Which of the following aids in recruitment of RNA polymerase to a promoter of a gene to initiate...
The lagging strand in DNA replication?: (A) is synthesized after the leading strand. (B) causes the...
The lagging strand in DNA replication?: (A) is synthesized after the leading strand. (B) causes the formation of Okazaki fragments in the leading strand. (C) is a consequence of replicating both strands of template DNA at a single replication fork. (D) requires its own replisome.
Match the following (Total 24 pts) Enzyme responsible for joining DNA strands together The nascent DNA strand that is being synthesized in the same direction as the replication fork. Enz 39 DNA polymerase 40 DNA helicase responsible for transcribing RNA 41DNA sliding clamp 42 Single Stranded Binding Proteins DShort, newly synthesized DNA fragments 43 RNA primer 44 DNA ligase formed on the lagging template strand E Abundle or proteins that assist in the rate of transcription of DNA to mRNA....
If the sequence of the 5'-3'DNA strand is AATGCTAC, then the complementary DNA sequence has the following sequence o 3-AATGCTAC-5' 3'-CATCGTAA-5 3-GTAGCATT-5 3-TTACGATG-5 Question 2 20 pts Which of the following does the enzyme primase make? phosphodiester linkages (bonds) Okazaki fragments RNA primer DNA primer In which direction does DNA replication take place? 3'to 5 5'to 5 5'to 3 3'to 3 Question 4 20 pts Which enzyme unwinds the double-stranded helical DNA starting at the origin of replication? ligase primase...
BioLoG 11. chose the order below that most closely represents the order in which the following proteins participate in DNA replication. a. helicase, single stranded binging protein, primase DNA polymerase b. single -stranded binding protein, primase DNA polymerase helicase c. primase, DNA polymerase, single- stranded binding protein, helicase d. helicase, single- stranded binding protein DNA polymerase, primase. 12. what is the function of the enzyme primase during DNA replication? a. to unwind the double helix to prime it for replication...
If the DNA polymerase builds both chains from the same fork, how is the second strand built if DNA polymerase only synthesizes DNA one direction (S' to 3)? The lagging strand is synthesized continuously using many short fragments, called Okazaki fragments. The leading strand is synthesized discontinuously using many short fragments, called Okazaki fragments. The lagging strand is synthesized discontinuously using many short fragments, called Okazaki fragments. The leading strand is synthesized discontinuously using many short fragments, called Okazaki fragments....
D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes the new strand of RNA primase Choose synthesizes the new strand of DNA synthesizes a short fragment of complementary RNA attach to template DNA strands to prevent hydrogen bonding Uncoils the supercoil of prokaryotic chromosomes connects Okazaki fragments of new lagging strand of DNA unwinds and unzips the double stranded DNA Single stranded binding proteins (SSB's) DNA Ligase [Choose] Helicase [Choose) DNA Gyrase (Choose]...
2. Draw replication fork, and place and label the following -DNA being copied (template)-new D - Correct 5' and 3' markings NA-helicase -primaseleading strand and Okazaki fragments
Question 25 4 pts Which of the following terms are associated with the discontinuously replicated strand during DNA replication? I. DNA ligase II. Okazaki fragments III. lagging strand IV. leading strand V. RNA polymerase O I, II, III O I, II, III, V O II, IV, V O I, II, IV, V O II, III, V 4 pts Question 26 4 pts Which of the following aids in recruitment of RNA polymerase to a promoter of a gene to initiate...
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