Suppose you are engineering gene Y such that when it is inserted into a eukaryotic chromosome, it will be expressed continuously. Which specific sequences must you include before this gene so that it will be expressed? In what type of location in the chromosome will it need to be placed?
To express the gene in the chromosome the promoter sequences are must before the gene for it to be expressed. Promoter sequence is the site where transcription of a particular gene starts.
There are two parts in chromosome one is euchromatin and the other one is heterochromatin. The euchromatin is the part of the chromosome that is lightly packed and contain over major portion of the genes in chromosomes. Euchromatin is the region that is in active transcription. And on the other hand the heterochromatin is dense packed and not active in transcription. So our engineered gene to be expressed we need to put it in the euchromatin locations in the chromosome.
Suppose you are engineering gene Y such that when it is inserted into a eukaryotic chromosome,...
Mic2 is a gene present on both the X and Y chromosome of humans. Suppose a mutant form, mic2, results in an abnormal surface antigen. Assume that it is possible to identify heterozygous and homozygous individuals. If you had a large enough pedigree, could one differentiate between autosomal and pseudoautosomal inheritance? Explain.
Mic2 is a gene present on both the X and Y chromosome of humans. Suppose a mutant form, mic2, results in an abnormal surface antigen. Assume that it is possible to identify heterozygous and homozygous individuals. If you had a large enough pedigree, could one differentiate between autosomal and pseudoautosomal inheritance? Explain.
nuclear transplantation site-specific recombination nuclear microinjection transposon cloning homologous repair ecdysone locus control region insulator sequences targeting germline chimeric/mosaic 1. In 1974, Rudolf Jaenisch created the first genetically modified animal, a mouse, by introducing novel genetic information into the Mus musculus Transgenic animals are often generated by , where DNA transgenes are inserted into the nucleus of a fertilized egg cells. These engineered eggs will give rise to progeny that possess one copy of the transgene and must be bred...
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5.) You have discovered mutants in two new gene mutants in Drosophila. One mutant, curl, has small, curly, nonfunctional wings. The other mutant, big, has enormous eyes. You decide to test if these two new genes are on chromosome #2, so you perform a three-gene linkage testcross using a gene that you know is on chromosome #2, trp, a mutant that cannot make its own tryptophan (and so must get it from its food). In all three...
5.) You have discovered mutants in two new gene mutants in Drosophila. One mutant, curl, has small, curly, nonfunctional wings. The other mutant, big, has enormous eyes. You decide to test if these two new genes are on chromosome #2, so you perform a three-gene linkage testcross using a gene that you know is on chromosome #2, trp, a mutant that cannot make its own tryptophan (and so must get it from its food). In all three cases, these mutant...
The Gumienny lab is trying to clone a gene that affects body size in the roundworm C. elegans. A mutation in this gene, called sma-12, makes small animals when homozygous. It was isolated in a screen that used the chemical mutagen ENU. sma-12 was mapped to Chromosome V (5). To more finely map the location to identify the causative mutation, they perform a two-factor mapping experiment with another Chromosome V-linked gene, unc-23, which has a known position on Chromosome V....
Imagine that you are a geneticist and that you are just starting your practice. To help your patients, please create a brochure on a genetic disorder that will be displayed in your waiting room. Your pamphlet must include: Name of the disorder -Sly Syndrome (Mucopolysaccharidosis Type VID) Describe the condition. How does it affect the body? Identify the gene on which the disorder is located and the chromosome on which the gene is located. Is the disorder a problem with...
For the next group of questions consider a diploid cell from a eukaryotic organism with a total of ten chromosomes. After one round of the cell cycle is complete you observe a total of four daughter cells. During this cell division occurred and the resulting daughter cells are. mitosis; haploid with ten total chromosomes each mitosis; diploid with five total chromosomes each meiosis; diploid with ten total chromosomes each meiosis; haploid with five total chromosomes each Before the cell divided,...
mitosis meiosis during which part of the cell cycle do you see replication of DNA? what type of cytoskeletal filament is on the spindle? when in mitosis do you see the seperation of chromatids? what do we call the location of a gene on the chromosome?
it is a molecular biology question and requires designing an experiment and it is not missing information. if we want to perform a ChiP-seq experiment in cell typeA to analyze genome-wide binding by favorite transcription activator (YFTA). I need to design an experiment that investigate if binding at one of the sites actually affects transcription. select one site that is located upstream of highly transcribed gene (HTGZ), which is expressed in cell typeA, but not expressed in cell type B....