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To find out the concentration of unknown substance, You have to extrapolate the standard curve as I have done.
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2. You take the OD of the diluted solution (from question 1), and get a value...
In lab, you diluted your unknown protein solution by combining 14 ?L of unknown protein solution...
In lab, you diluted your unknown protein solution by combining
14 ?L of unknown protein solution with enough
PBS buffer to obtain a total volume of 100 ?L, then added 1000 ?L Bradford reagent. After waiting ten
minutes, the absorbance at 595 nm was 0.206. Using the standard
curve from the previous question, calculate the total concentration
of protein (in ?gmL) in the original sample.
Slope = 0.02371 Intercept = 0.01132 r2
= 0.973
You have a protein solution. You take 5 ml of the solution, and add 25 ml...
You have a protein solution. You take 5 ml of the solution, and add 25 ml of buffer. You then add 50 µl of the dilute protein solution to 50 µl of buffer and 2 ml of Coomassie solution. The resulting absorbance at 595 nm was 0.457. At the same time, you prepared a standard curve for the same protein using the same Coomassie assay protocol (100 µl of sample + 2 ml of coomassie reagent) The linear fit to...
Please answer #2. I'm unsure how its done ete od SOCK Solutions (the concentrations are known...
Please answer #2. I'm unsure how its done
ete od SOCK Solutions (the concentrations are known you prepare will be used in assessing concent (0.07wv) to be used in developing a spectrophotometric assay for this drug. The standard curve your disposal a primary standard solution of the drug in sal w be used in assessing concentrations of the drug in samples having unknown concentrations. You have at this STD). The linear range of the assay for this drug a primary...
Question 4 (2 points) A working standard 1 is prepared by taking 24.00 mL of a...
Question 4 (2 points) A working standard 1 is prepared by taking 24.00 mL of a 500.0 ppm stock solution and diluting it to a final volume of 360.0 mL. Working standard 2 is prepared by taking 3.00 mL of working standard 1 and diluting it to a final volume of 250.0 mL. Calculate the ppm concentration of the working standard 2. Provide your answer to three places after the decimal and without units. Your Answer: Answer Question 5 (2...
lab question 1. What is the basis of the different purification methods? 2. What are some...
lab question 1. What is the basis of the different purification methods? 2. What are some of the factors the might have interfered with your results? 3. How might you improve the process to increase the yield and purity? lab process E. coli BL21 (DE3) cells were transformed with the pET Topo-1521 vector containing a reading frame encoding the green fluorescent protein (GFP). Cells were cultured in M9ZB media at 37°C until the absorbance at 600 nm reached 0.7, at...
1. If a solution absorbed blue-green light, what color would the solution appear to be? Hint:...
1. If a solution absorbed blue-green light, what color would the solution appear to be? Hint: Table I, at the beginning of the experiment, will help you answer this one. 1. Calculate the concentration of a solution prepared by adding 15.00 mL of 2.09 ~ 10-3M KMnO4 from a buret into a 50.00 mL volumetric flask, which is then filled to the 50.00 ml graduation mark with distilled water. Hint: M 2. A linear standard curve of KMnO4 is prepared...
Can you help me answer 4&5? Zinc(ll) and the ligand L forma 1:1 complex that absorbs...
Can you help me answer 4&5?
Zinc(ll) and the ligand L forma 1:1 complex that absorbs strongly at 600 am As long as the molar concentration of L exceeds that of Zn(II) by a factor of 5. the absorbance depends only on the cation concentration Neither Zn(ll) nor L absorbs at 600 nm A solution that is 1.59 times 10^-4 M Zn(ll) and 1.00 times 10^-3 M in L has an absorbance of 0,352 in a 1.00-cm cell at 600...
1. Determining the Wavelength of Maximum Absorption: 2. Determining the concentration of an Unknown Solution Volume...
1. Determining the Wavelength of Maximum Absorption: 2. Determining the concentration of an Unknown Solution Volume of Stock Concentration of Soln #1 T2.001.xlon #2 5.00 2.5x loin . . x ^ 10.00 Soxom A=493.6 mm Rad y o sxlom Recorded Absorbance 0.212 0.528 0.947 1453 #4 Calibration Curve Slope of Least Squares Best Fit Line (molar abspoptivity, E) Equation for Least squares best fit line: A-42. Ubxk 2916xlor 6-01482 0.9652 Correlation Factor (R) for Least Squares Best Fit Line 3....
4) You have been given the job of designing an experiment to test the effects of...
4) You have been given the job of designing an experiment to test the effects of an inhibitor on the coronavirus polymerase. Assume you have a stock solution containing purified polymerase protein. You want to know how much polymerase protein you have in the stock solution. To do this, you will use the Bradford Assay. If you mix 50L of polymerase protein stock solution with 100L of water, perform the Bradford Assays and get a resulting OD 595mm of 0.6,...
1. You want to prepare a 1:50 dilution of your protein extract in a total volume...
1. You want to prepare a 1:50 dilution of your protein extract in a total volume of 1000 uL. You will need ___ uL of protein extract and ___ uL of water. 2. You are provided with a solution of BSA that is 100 ug/mL and make a 10-2 dilution? What is the resulting concentration of the DILUTED BSA? 3. Which of the following statements about enzymes is true? Select one: a. Enzymes increase the rate of a chemical reaction...
In lab, you diluted your unknown protein solution by combining
14 ?L of unknown protein solution with enough
PBS buffer to obtain a total volume of 100 ?L, then added 1000 ?L Bradford reagent. After waiting ten
minutes, the absorbance at 595 nm was 0.206. Using the standard
curve from the previous question, calculate the total concentration
of protein (in ?gmL) in the original sample.
Slope = 0.02371 Intercept = 0.01132 r2
= 0.973
Please answer #2. I'm unsure how its done
ete od SOCK Solutions (the concentrations are known you prepare will be used in assessing concent (0.07wv) to be used in developing a spectrophotometric assay for this drug. The standard curve your disposal a primary standard solution of the drug in sal w be used in assessing concentrations of the drug in samples having unknown concentrations. You have at this STD). The linear range of the assay for this drug a primary...
Question 4 (2 points) A working standard 1 is prepared by taking 24.00 mL of a 500.0 ppm stock solution and diluting it to a final volume of 360.0 mL. Working standard 2 is prepared by taking 3.00 mL of working standard 1 and diluting it to a final volume of 250.0 mL. Calculate the ppm concentration of the working standard 2. Provide your answer to three places after the decimal and without units. Your Answer: Answer Question 5 (2...
1. If a solution absorbed blue-green light, what color would the solution appear to be? Hint: Table I, at the beginning of the experiment, will help you answer this one. 1. Calculate the concentration of a solution prepared by adding 15.00 mL of 2.09 ~ 10-3M KMnO4 from a buret into a 50.00 mL volumetric flask, which is then filled to the 50.00 ml graduation mark with distilled water. Hint: M 2. A linear standard curve of KMnO4 is prepared...
Can you help me answer 4&5?
Zinc(ll) and the ligand L forma 1:1 complex that absorbs strongly at 600 am As long as the molar concentration of L exceeds that of Zn(II) by a factor of 5. the absorbance depends only on the cation concentration Neither Zn(ll) nor L absorbs at 600 nm A solution that is 1.59 times 10^-4 M Zn(ll) and 1.00 times 10^-3 M in L has an absorbance of 0,352 in a 1.00-cm cell at 600...
1. Determining the Wavelength of Maximum Absorption: 2. Determining the concentration of an Unknown Solution Volume of Stock Concentration of Soln #1 T2.001.xlon #2 5.00 2.5x loin . . x ^ 10.00 Soxom A=493.6 mm Rad y o sxlom Recorded Absorbance 0.212 0.528 0.947 1453 #4 Calibration Curve Slope of Least Squares Best Fit Line (molar abspoptivity, E) Equation for Least squares best fit line: A-42. Ubxk 2916xlor 6-01482 0.9652 Correlation Factor (R) for Least Squares Best Fit Line 3....
4) You have been given the job of designing an experiment to test the effects of an inhibitor on the coronavirus polymerase. Assume you have a stock solution containing purified polymerase protein. You want to know how much polymerase protein you have in the stock solution. To do this, you will use the Bradford Assay. If you mix 50L of polymerase protein stock solution with 100L of water, perform the Bradford Assays and get a resulting OD 595mm of 0.6,...
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