Question

You are studying a human protein, XYZ, that localizes to the nucleus in human cells. You want to see if it will also localize to the nucleus in mouse cells. To do this, you clone a translational fusion of the jellyfish fluorescent protein, called GFP, to XYZ and put it into a plasmid. This means that XYZ and GFP are now translated as a single protein. Since XYZ is now fused to GFP, you can simply image GFP to see if it has gone into the nucleus, and if it has, you know XYZ has also. To do this experiment, you transform your plasmid encoding the XYZ-GFP fusion gene into E. coli so that you can produce a lot of the plasmid. You then harvest the plasmid and use CRISPR/Cas9 technology to trick homologous recombination into copying your XYZ-GFP fusion gene into a defined site in the mouse genome. You then image the mouse cells. Which organisms must recognize the promoter driving the XYZ-GFP fusion gene for this experiment to work? Only jellyfish Only E.coli Only mouse Only human Jellyfish and E.coli E.coli and mouse Mouse and human E.coli, mouse, and human Jellyfish, E. coli, mouse, and human

Answer 1

Answere :-

Correct option is "only mouse"

Explanation :- aim of given experiment is to study the human protein XYZ , whether it localises in mouse nucleus as it localises in human cells.

For that we fused this protein with jellyfish flueroscent protein -GFP.

Then we put it to plasmid , where it translated as a single protein (this step occured outside the human and jellyfish cells).

This plasmid is then inserted to E.coli to obtain more plasmid.

(Plasmid is a self replicating organelle therefore Ecoli doesn't required a promoter driving given fused gene)

It is then harvested,and by using crisper /cas9 technology to trick homologous recombination into copying this fused gene into a defined site in the mouse gene.(here mouse cell required the promoter driving XYZ-GFP gene ).