Question

the genetic blueprint of an organism is referred to as i

A scientist wishes to genetically engineer E. coli bacteria so that it will contain the green fluorescent protein (GFP) gene. Which of the following protocol outlines would the scientist use to accomplish this task? ОА Insert GFP gene into corn expression cassette; insert expression cassette into plasmid vector; insert plasmid vector into Agrobacterium tumefaciens bacteria; allow bacteria to infect corn plant; GFP gene inserted into com cell genome. . Isolate fibroblasts from human and grow them in culture; insert functional gene into a virus or other vector, add vector to fibroblast culture and allow vector to insert functional gene into fibroblast DNA, culture these genetically engineered cells and re-inject them into the patient to produce the missing protein. Isolate the human growth hormone gene from human DNA by cutting with restriction enzymes; isolate plasmid DNA and cut it with restriction enzymes; mix the DNAs together allowing the sticky ends to join and yield a recombinant plasmid with human DNA coding for human growth hormone; insert the plasmid into E.coli; let the E coli grow and divide and produce human growth hormone. Isolate GFP gene from bloluminescent jelly fish, Aequorea victoria and cut with restriction enzyme; insert functional gene into a virus or other vector; add vector to fibroblast culture and allow vector to insert functional gene into fibroblastic DNA of E. coli; let E. coli grow and divide and produce GFP. Isolate GFP gene from bioluminescent jellyfish, Aequorea victoria cutting it with restriction enzyme; isolate a nisemin from E. coli cuttine it with restriction enzyme: mix the DNAs together, allowing sticky ends to join 3 Fulls

Answer 1

D. isolate GFP gene from bioluminescent jelly fish, Aequora victoria and cut with restriction enzyme, insert functional gene into a virus or other vector; added vector to fibroblast culture and allow vector to insert functional gene into fibroblast DNA of E.coli; let E.coli grow and divide and produce GFP.

The above technique can be followed to genetically engineer E.coli bacteria so that it will contain the Green fluorescent protein gene.