Question

Explain how you might use E. coli bacteria to produce human growth hormone using the following: plasmid, b. Gene segment for human growth hormone that contains only exons, DNA ligase, Restriction enzymes, and Incubator for growing bacteria cells.

Answer 1

E.coli can produce human growth hormone by insertion of human gene segment in to bacteria using transformation process

E.coli contain plasmid which is extra chromosomal material and we can insert or delete some segment of DNA in plasmid without affecting bacteria cell life.

and this help us to produce human proteins or hormones in bacteria

so the process of insertion and expression of human gene in bacteria as follows

isolate Gene segment for human growth hormone that contains only exons (because bacteria can not remove introns from human gene mRNA and it will affect translation process) ------->cut plasmid and human gene with same restriction enzyme(so that it produce same sticky ends )-----> ligate gene and plasmid with DNA ligase enzyme---->transfer recombinant plasmid in to bacterial cell ----> incubate bacteria cell in incubator for proper growth.-----> isolate/purify human hormone

if recombination and transfer is successful of plasmid then gene in plasmid will express in bacteria and produce hormone which is purify from bacteria cell/ broth