Question

RECOMBINANT DNA: PLASMID VECTOR engineering is the direct manipulation of an organism's DNA using nology. To begin the recombinant DNA process, scientists must first ide at codes for the production of the protein they want to manufacture. One is to go backwards from the amino acid sequence of the desired protein to ide sequence of the gene. After scientists have identified the gene, they m it. Restriction enzymes or endonucleases from bacterial cells are key in th ia produce restriction enzymes to cut down foreign DNA. Many restriction es cut the DNA leaving sticky ends, single strands of nucleotide bases capa ng with complementary sticky ends. By using enzymes that cut the DNA on -f the gene, the gene can be cut out of the cellular DNA strand. To get the DN est into the host cell, it has to be inserted into a plasmid, which serves as the v ansformation. If the plasmid is cut with the same restriction enzyme as the gei est, the sticky ends left by the restriction enzyme will be complementary. The be inserted into the plasmid by joining (ligating) the nicks in the sugar-phospha DLA with DNA ligase. The next step is the transform identification of positive clones or ce selective

Answer 1