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You successfully inserted a foreign piece of DNA, e.g. the gene for human erythropoetin (EPO), into...

You successfully inserted a foreign piece of DNA, e.g. the gene for human erythropoetin (EPO), into a bacterial plasmid, which also contains a gene conferring antibiotic resistance to ampicillin. What is your next step you do on the way to finally produce the human hormone in E.coli?
              A) you cut the plasmid with restriction enzymes
              B) you grow E.coli on agar plates containing ampicillin
              C) you grow E.coli on agar plates containing tetracycline
              D) you insert the recombinant plasmid into E.coli, i.e. you transform E.coli

E) you grow E.coli on agar plates containing ampicillin

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Answer #1

Ans:The next step in the recombinant DNA technology is always transferring this recombinant plasmid to host i.e bacteria E.coli so option D is correct.

After that transfer the bacteria containing the recombinant would be allowed to grow on Agar plate containing ampicillin.

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