Question

Design Layout References Mailings Review View Help Picture Format B. Show the band (s) consisting of highest number of base pairs in Hindill and Hindill Xbol lane. C. Show the band (s) consisting of lowest number of base pairs in Hindi and Hindi Xbollane Part 2. The following picture is landa DNA with Hind Ill cut sites are show in the map. The numbers are showing frements sizes following Hind 11 digestion, for example 23 1 a 23.1 Kb fragment that will be generated as one of the fragment following Hindill digestion of the lamda DNA a) Based on this map lahel all the fragment in the Hindi lane of the gel. b) Based on the pattern of bands/ band sizes in the Xholt Hind III lane. out Xhol cut sit in the map

ok so this is a practice problem and I just want to make sure its correct. the first pic is the whole question the the second is the pic in the second part of the question. the third pic is the same pic in the first and second part of this question I just wanted to make sure the picturs are big enough to see thats why I uploaded them. If you have a problem please let me know.

Answer 1

Answer:

The whole Lambda DNA is a linear DNAa has the total base pair 48.5kb

On HindIII restriction digestion only, the Lambda DNA cuts into total 8 pieces i.e. the Lamda DNA has total 7 restriction sites of HindIII. The cut pieces sizes are as follows; 23.1kb, 9.4kb, 6.6kb, 4.4kb, 2.3kb, 2.0kb, 0.6kb and 0.1kb

Upon XhoI digestion only, the Lamda DNA cuts into two pieces i.e. it has one restriction site for XhoI restriction enzyme. 33.5kb and 15kb

Upon double digestion with HindIII and XhoI, the 9.4kb fragment breaks into 6kb & 3.4kb. This can be seen in agarose gel in the double digestion well.