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2) What is the significance of the mycolic acid layer in the following contexts? A) To...
QUESTIONS 1) Are acid fast cells gram positive or gram negative? Explain why it might be...
QUESTIONS 1) Are acid fast cells gram positive or gram negative? Explain why it might be difficult to determine the Gram reaction of acid fast cells. 2) What is the significance of the mycolic acid layer in the following contexts? A) To bacteria for survival and infection B) During the acid fast staining procedure QUESTIONS 1) What is the difference between a pure culture and a pure colony? Is a streaking for isolation plate a pure culture when your original...
Lab Supplemental Questions hital Questions Acid-Fast Stain 1. Why is acid-alcohol used as the decoloriting agent...
Lab Supplemental Questions hital Questions Acid-Fast Stain 1. Why is acid-alcohol used as the decoloriting agent in the dio Aud-alcohol is used as the decolorizing Staining because it ensures that the pro form non acld fast organisme. agent in the acid-fast stain? orizing agent in the acid-fast the primary stain is removed 2. Why don't we Gram stain the mycobacterium? stain both acid-fast and non-acid-fast bacteria stain red, what 3. If at the end of the acid-fast stain both acid-fast...
Lab Report: 6 Acid-Fast Staining 1 What is the purpose of the acid-fast staining technique? To...
Lab Report: 6 Acid-Fast Staining 1 What is the purpose of the acid-fast staining technique? To Identity acil fast and on need fout bacteria To descrote the inhocal sigraf cance of acid-foot staining 2. Complete the following table with your observations Name of the Bacteria Color of the cells Acid-last/ non acid-last Drawing (color) 3. What might account for the fact that some bacteria are acid-fast and others are non acid-fast? What is the clinical significance of determining whether or...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order,...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
What two historically important diseases are caused by acid fast bacteria? Give the scientific name for...
What two historically important diseases are caused by acid fast bacteria? Give the scientific name for each of the organisms that cause these diseases. 2. 3. How does acid-fast stain detect mycolic acids? 4. Are there species from Mycobacterium genus that are part of the normal flora? If so, write the name and function.
What color would ine bacterial cells appear if the patient has tuberculosis? and capsule stains The...
What color would ine bacterial cells appear if the patient has tuberculosis? and capsule stains The only difference is that without heat you have to stain the smear with carbol fuschin for 10 1. What is chemically unique about the Mycobacterium genus that personal Note: Alternate cold method acid fast stain (Kinyoun) minutes My note: There is not only one difference. The counterstain here is brilliant green rather than methylene blue And to allow the carbol fuchsin to penetrate acid...
Why acid-fast bacteria can resist decolorization by acids during laboratory staining?
Why acid-fast bacteria can resist decolorization by acids during laboratory staining?
BACKGROUND: Mycobacterium tuberculosis causes tuberculosis, one of the world’s deadliest diseases...
BACKGROUND:
Mycobacterium tuberculosis causes tuberculosis, one of the world’s
deadliest diseases. It is an acid-fast bacillus that is 2-4 um in
length and 0.2-0.5 um in width.
How many weeks, days,& hours would it take to see a
M.tuberculosis colony on solid media? Its generation time is listed
in the reading (above). Assume that bacterial colonies are not
visible to the naked eye unless they contain ≥ 2 millioncells. This
species forms aggregates of 5 cells on average. Show your...
Questions What does the term "Acid Fa" mean? Are acid-Fast organisms Gram-positive or Gram- 2 Do...
Questions What does the term "Acid Fa" mean? Are acid-Fast organisms Gram-positive or Gram- 2 Do your results indicate that endospores are acid-fast? Explain. 3 Why is it best to use "old" cultures for this staining procedure? 4 When you stain your Mycobacterium smegmatis, some of the cells are red and others are blue. What does tell you about your culture?
LAB REPORT 2- Selected questions from Exercise 3-7 (Acid-fast stain) Note: This is turned in after...
LAB REPORT 2- Selected questions from Exercise 3-7 (Acid-fast stain) Note: This is turned in after you have viewed your acid fast stain slides. Bacteria for this exercise are: Mycobacterium smegmatis (or M. phlei) and S. aureus. Q1. Acid fast bacteria are have some significant differences as compared to non-acid fast bacteria. What are the major chemical (structural) differences among these different bacteria that might explain the acid-fast stain? Hint: This structural difference also affects the way the bacteria grow...
QUESTIONS 1) Are acid fast cells gram positive or gram negative? Explain why it might be difficult to determine the Gram reaction of acid fast cells. 2) What is the significance of the mycolic acid layer in the following contexts? A) To bacteria for survival and infection B) During the acid fast staining procedure QUESTIONS 1) What is the difference between a pure culture and a pure colony? Is a streaking for isolation plate a pure culture when your original...
Lab Supplemental Questions hital Questions Acid-Fast Stain 1. Why is acid-alcohol used as the decoloriting agent in the dio Aud-alcohol is used as the decolorizing Staining because it ensures that the pro form non acld fast organisme. agent in the acid-fast stain? orizing agent in the acid-fast the primary stain is removed 2. Why don't we Gram stain the mycobacterium? stain both acid-fast and non-acid-fast bacteria stain red, what 3. If at the end of the acid-fast stain both acid-fast...
Lab Report: 6 Acid-Fast Staining 1 What is the purpose of the acid-fast staining technique? To Identity acil fast and on need fout bacteria To descrote the inhocal sigraf cance of acid-foot staining 2. Complete the following table with your observations Name of the Bacteria Color of the cells Acid-last/ non acid-last Drawing (color) 3. What might account for the fact that some bacteria are acid-fast and others are non acid-fast? What is the clinical significance of determining whether or...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
What two historically important diseases are caused by acid fast bacteria? Give the scientific name for each of the organisms that cause these diseases. 2. 3. How does acid-fast stain detect mycolic acids? 4. Are there species from Mycobacterium genus that are part of the normal flora? If so, write the name and function.
What color would ine bacterial cells appear if the patient has tuberculosis? and capsule stains The only difference is that without heat you have to stain the smear with carbol fuschin for 10 1. What is chemically unique about the Mycobacterium genus that personal Note: Alternate cold method acid fast stain (Kinyoun) minutes My note: There is not only one difference. The counterstain here is brilliant green rather than methylene blue And to allow the carbol fuchsin to penetrate acid...
BACKGROUND:
Mycobacterium tuberculosis causes tuberculosis, one of the world’s
deadliest diseases. It is an acid-fast bacillus that is 2-4 um in
length and 0.2-0.5 um in width.
How many weeks, days,& hours would it take to see a
M.tuberculosis colony on solid media? Its generation time is listed
in the reading (above). Assume that bacterial colonies are not
visible to the naked eye unless they contain ≥ 2 millioncells. This
species forms aggregates of 5 cells on average. Show your...
Questions What does the term "Acid Fa" mean? Are acid-Fast organisms Gram-positive or Gram- 2 Do your results indicate that endospores are acid-fast? Explain. 3 Why is it best to use "old" cultures for this staining procedure? 4 When you stain your Mycobacterium smegmatis, some of the cells are red and others are blue. What does tell you about your culture?
LAB REPORT 2- Selected questions from Exercise 3-7 (Acid-fast stain) Note: This is turned in after you have viewed your acid fast stain slides. Bacteria for this exercise are: Mycobacterium smegmatis (or M. phlei) and S. aureus. Q1. Acid fast bacteria are have some significant differences as compared to non-acid fast bacteria. What are the major chemical (structural) differences among these different bacteria that might explain the acid-fast stain? Hint: This structural difference also affects the way the bacteria grow...
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