Homework Answers
Answer: If the glucose tube is yellow that means glucose is fermented. The presence of bubble indicates gas production.
The lactose and sucrose tubes are not showing any colour change means the organism is not able to ferment lactose and sucrose.
Sugar fermentation is an important criteria to distinguish between bacteria. The positive fermentation can be detected by color change in the medium, phenol red is used as an indicator which turns yellow in acidic conditions.
This test is used to distinguish between Gram negative enteric bacteria. To confirm presence of a particular type of organisms the results should be compared with inoculated/uninoculated controls. Further identification procedure needs to be done to ascertain a particular type of bacteria.
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5. In Exp. 22, if your glucose fementation tube is yellow and produces a bubble and...
phenol red broth 5-3 5-3 Enter your results color and bubble/no include the relevant enzyme's name...
phenol red broth 5-3
5-3 Enter your results color and bubble/no include the relevant enzyme's name in your Phenol Red Broth OBSERVATIONS AND INTERPRETATIONS color and bubble/no bubble in the table below. using the symbols shown in Table 5-2, page 305. Be sure to vant enzyme's name in your interpretation is an organism ferments sucrose and/or lactose Organism PR Base Results PR PR Glucose Lactose PR Sucrose Interpretation Uninoculated control LAC GLU E. Colin LAC SUC GLU Proteus vulgaris LAC...
Microbiology Q1 Q2 Q3 A phenol red glucose broth with a Durham tube has been inoculated...
Microbiology
Q1
Q2
Q3
A phenol red glucose broth with a Durham tube has been inoculated and incubated. After 48 hours, the broth is turbid and bright yellow and approximately half of the contents of the Durham tube contains bubbles. Interpret these results. The organism will ferment lactose as well. The organism is fermenting glucose. The organism is an obligate anaerobe. The organism is fermenting lactose and producing ethanol and CO2. What can be detected using SIM medium? Check all...
Microbiology Q1 Q2 Q3 Which medium contains a pH indicator to show pH change caused by...
Microbiology
Q1
Q2
Q3
Which medium contains a pH indicator to show pH change caused by fermentation? Glucose Broth Lactose Broth SIM Lactose and Glucose Broths Lactose, Glucose, and SIM What is the Gram reaction of Escherichia coli? Positive Negative Gram Neutral It is an acid-fast organism. How do we test for gas production during glucose fermentation? The medium turns yellow. There is a bubble that collects in the Durham tube. The medium turns red. We cannot test for gas...
Background Background continued... Questions Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram...
Background
Background continued...
Questions
Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
please answer questions 1 through 6 with the information provided. Enzyme Specificity Are Sucrase and Lactase...
please answer questions 1 through 6 with the
information provided.
Enzyme Specificity Are Sucrase and Lactase specific to Sucrose and Lactose? I: Experimental Procedure 1. Add 0.3 g of sucrose to three different test tubes. Make sure to remember to label them 1 to 3. 2. Add 5 mL of Dl water to test tube number 1. Add 5 mL of sucrase to test tube 2. Add 5 ml of lactase to test tube 3. 3. Add 0.3 g of...
I need help creating a flowchart on a unknown 89 Chart of Unknown Test and the...
I need help creating a flowchart on a unknown 89 Chart of Unknown Test and the Result #89 Name of Test What does it test? Positive result appears as: negative results appears as: Unknown #89 result: Gram Stain Reaction Cell envelope morphology Violet-blue Red Negative (pink) Cell morphology (shape: coccus, bacillus, or spirillum) Cell shape Bacillus (Rod shaped) Cell Arrangement Cell size or length Single Bacillus Pigment Cell envelope morphology Pink Oxygen Requirement If cell needs oxygen for respiration Growth...
yellow, green or red, the test is positive. The color of the solution depends on the...
yellow, green or red, the test is positive. The color of the solution depends on the concentration of 3. Observe the color of your negative and positive controls. Note which one has a red color. 4 After the dialysis of the NaCi-elucose-red dye solution has progressed for an hour, test the water outside the dialysis bag (your experimental data) for the presence of Ci and glucose same way you did for your controls in steps 1 and 2. Observe if...
4&5 please 4. Juan is a new lab tech who needs to inoculate agar slants that...
4&5 please
4. Juan is a new lab tech who needs to inoculate agar slants that students will be using to make endospore stain smears. He inoculates the tubes on Monday for a lab the following day. Did the students find endospores on their slides? Explain why or why not. 1 point 5. After inoculating with her unknown, Letitia's Phenyl Red Glucose, Lactose, and Sucrose tubes are all yellow. Her TSI tube results are K/H2S. The EMB plate result is...
Assuming, as in Question 1. you tested an organism using PR Glucose and PR Base broths,...
Assuming, as in Question 1. you tested an organism using PR Glucose and PR Base broths, which of the combi nations of results in the following table would be reliable. Interpret each of the combinations and explain how the results might be obtained. PR PR Base Reliable Y/N Glucose A/G AIG Interpretation fermentation with acid and gas end products in PR base Hermentation with acid end products In PR glucoses no ferm. in Pr buse fermentation w/ acid s gas...
14. Lab Exercise 5-1 is an interesting way to run these test tube experiments without oxygen...
14. Lab Exercise 5-1 is an interesting way to run these test tube experiments without oxygen - seal the tube with a layer of clear mineral oil on top (see the figure in the manual). Based on this figure and the Table of Results for this experiment, if the sealed tube is all green or slightly blue but the unsealed tube has any amount of yellow, what type of energy metabolism does that organism have? a. Oxidative only b. Oxidative...
phenol red broth 5-3
5-3 Enter your results color and bubble/no include the relevant enzyme's name in your Phenol Red Broth OBSERVATIONS AND INTERPRETATIONS color and bubble/no bubble in the table below. using the symbols shown in Table 5-2, page 305. Be sure to vant enzyme's name in your interpretation is an organism ferments sucrose and/or lactose Organism PR Base Results PR PR Glucose Lactose PR Sucrose Interpretation Uninoculated control LAC GLU E. Colin LAC SUC GLU Proteus vulgaris LAC...
Microbiology
Q1
Q2
Q3
A phenol red glucose broth with a Durham tube has been inoculated and incubated. After 48 hours, the broth is turbid and bright yellow and approximately half of the contents of the Durham tube contains bubbles. Interpret these results. The organism will ferment lactose as well. The organism is fermenting glucose. The organism is an obligate anaerobe. The organism is fermenting lactose and producing ethanol and CO2. What can be detected using SIM medium? Check all...
Microbiology
Q1
Q2
Q3
Which medium contains a pH indicator to show pH change caused by fermentation? Glucose Broth Lactose Broth SIM Lactose and Glucose Broths Lactose, Glucose, and SIM What is the Gram reaction of Escherichia coli? Positive Negative Gram Neutral It is an acid-fast organism. How do we test for gas production during glucose fermentation? The medium turns yellow. There is a bubble that collects in the Durham tube. The medium turns red. We cannot test for gas...
Background
Background continued...
Questions
Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
please answer questions 1 through 6 with the
information provided.
Enzyme Specificity Are Sucrase and Lactase specific to Sucrose and Lactose? I: Experimental Procedure 1. Add 0.3 g of sucrose to three different test tubes. Make sure to remember to label them 1 to 3. 2. Add 5 mL of Dl water to test tube number 1. Add 5 mL of sucrase to test tube 2. Add 5 ml of lactase to test tube 3. 3. Add 0.3 g of...
yellow, green or red, the test is positive. The color of the solution depends on the concentration of 3. Observe the color of your negative and positive controls. Note which one has a red color. 4 After the dialysis of the NaCi-elucose-red dye solution has progressed for an hour, test the water outside the dialysis bag (your experimental data) for the presence of Ci and glucose same way you did for your controls in steps 1 and 2. Observe if...
4&5 please
4. Juan is a new lab tech who needs to inoculate agar slants that students will be using to make endospore stain smears. He inoculates the tubes on Monday for a lab the following day. Did the students find endospores on their slides? Explain why or why not. 1 point 5. After inoculating with her unknown, Letitia's Phenyl Red Glucose, Lactose, and Sucrose tubes are all yellow. Her TSI tube results are K/H2S. The EMB plate result is...
Assuming, as in Question 1. you tested an organism using PR Glucose and PR Base broths, which of the combi nations of results in the following table would be reliable. Interpret each of the combinations and explain how the results might be obtained. PR PR Base Reliable Y/N Glucose A/G AIG Interpretation fermentation with acid and gas end products in PR base Hermentation with acid end products In PR glucoses no ferm. in Pr buse fermentation w/ acid s gas...
14. Lab Exercise 5-1 is an interesting way to run these test tube experiments without oxygen - seal the tube with a layer of clear mineral oil on top (see the figure in the manual). Based on this figure and the Table of Results for this experiment, if the sealed tube is all green or slightly blue but the unsealed tube has any amount of yellow, what type of energy metabolism does that organism have? a. Oxidative only b. Oxidative...
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