Question

1.

In the following double-stranded DNA sequences, ___ has the lowest melting temperature and __ has the highest melting temperature.

     1) GATCG 2) GGCCG 3) AATTA 4) AATCA

        CTAGC    CCGGC    TTAAT    TTAGT

2.

In linear nucleic acids, nucleotides are bonded through their ___ and ___ carbon atoms.

3.

Although RNA and DNA are similar in structure, one difference is that ___.

		DNA uses the base adenine
		RNA uses the base cytosine
		the RNA sugar ring bears a 2’OH
		the DNA sugar ring bears a 3’OH

Answer 1

1: To Calculate melting temperature for DNA sequence < 14 nucleotides - Number of nucleotides A For sequence GATCG TG-2, A-1, T-1, 6-1) CTAGE L. Melling temperature = 4(+c) + 2 (A+T) = 4(2+1) + 2 (1+1) 3 4x3 + 2x2 = 1274 = 168 By for sequence GGCCG [ 6-3, 6-27 CC GGze L Melting temperature = 4(G+e) + 2 (A+T) = 4(3+2) + 2(6+0) = 4x5 to = 20°c TTAAT y for sequence. AATTA Į A-3, 7-2] Melting temperature = 4(G+e) + 2(A+T) = (0+0) + 2(3+2) = 0 + 2x5 € 10°C D) from sequence - ARTEA TA-3,5-1, 6-3) TTAGET L Melting temperature = 4(6+C) + 21 A+T) = 4( 0+1) +2(3+) Higher the member of Geone 7 = 4x 1 + 2x4 nucleotides, higher the melting temp = 4 + 8 = 12°c sequene-2 has high melting temperature and sequence - 3 has low melting temperature Answer

1 n. In Linear nucleic acido, nucleotides are bonded together through their 5 and 3 Carbon atoms. Explanation - when the nucleotides which are linear become bonded with the help of 5 and 3' carbon atom, then linear nucleic acid formed . The 5' Carbon atom and 3' Carbon atom are linked through Phospho di-ester bond. Answer the RNA sugar ring beans a 20h Explanation - DNA has devotees DNA has de-oxg ribose sugar and forura nucleotides Adenine, Guanine, cytosine and thy mine. RNA has ribose sugar and four nucleotides Adenine, Guanine, cytosine, wracil. CH2OH O. OH 3CH2OH I/O OH t C'H HĆ l' TN 1/1 2 H H &T et OH H 예 애 3 OH Be-oxyribose sugar Ribose sugar. .. De-oxyribose sugar and Ribose segar both have 3'OH 2. So, the RNA sugar ring bears 20h [Asiswer] Sugar Do.