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for the test tubes that had protein digestion, what needs to occur to get the test...

for the test tubes that had protein digestion, what needs to occur to get the test tubes to full digestion? (a clear tube)?
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In order to ensure complete digestion of proteins, a proteolytic enzyme such as pepsin may be used. However, this enzyme requires appropriate temperature and a low (acidic) pH to be active. Thse conditions in a test tube will replicate the conditions in a mammalian stomach where in presence of acidic conditions(HCL, pH=1.5-3.5) and the proteolytic enzyme, pepsin. Further, the human body temperature is 37 degree celsius and thus, the test tube must be incubated at a similar temperature. Once all these conditions are met, complete digestion of proteins will occur and a clear tube will be obtained.

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