Question

MICROBIOLOGY HELP

Exercise: Understanding restriction enzyme digests 1. Below is an image of a plasmid. Various parts of the pl location of two restriction enzyme sites fiction enzyme sites (EcoRI and Hindill) are shown. id. Various parts of the plasmid as well as the ECOR PYFG drugR A researcher performs some restriction enzyme digests of this plasmid. In separate tubes, he digests the plasmid with EcoRI alone. HindIll alone, and EcoRI and Hindill together. He also sets up a sample of plasmid without any enzyme to use as an uncut control. He runs each of his samples in a separate lane of an agarose gel. Below is his gel image. Approximately how large (in base pairs) is the YFG gene? Hindi EcoRI/Hindi

Answer 1

EcoRI cuts at only one site and HindIII also cuts at one site, EcoRI cuts at one end of the YFG gene and HindIII cuts at another end of the YFG gene, the smallest fragment obtained in the combined digest of EcoRI and HindIII corresponds to the YFG gene. the length of smallest band in the combined digest of EcoRI and HindIII is 950 bp long so the size of the YFG gene is 950 bp.