Question

1- In addition to the DNA building blocks (nucleotides dATP, dCTP, dGTP, dTTP) the master mix contain:

A) Taq DNA polymerase

B) Buffer

C) Magnesium chloride

D) Specific primers to amplify the BXPOO7 locus

E) All of the above

2- PCR partially simulates ‘in vivo”

A) RNA synthesis

B) DNA transcription to RNA

C) Protein synthesis

D) DNA replication

Answer 1

E) All of the above . Taq DNA polymerase will polymerize the nucleotides , buffer will provide optimum pH conditions for the enzyme to work , MgCl2+ act as a cofactor for Taq polymerase and primers will help in specific amplification of BXPOO7 .

2) DNA replication . Beacuse new copy of a specific DNA sequence is generated in vitro.