A linear DNA molecule was digested with restriction enzyme EcoR I (E) and three fragments were...
A linear DNA molecule was digested with restriction enzyme EcoR I (E) and three fragments were produced of sizes 7.3 kb, 4.0 kb and 3.4 kb. The restriction enzyme BamH I (B) produced three fragments of sizes 7.9 kb, 4.8 kb and 2 kb. When the two enzymes EcoR I and BamH I were used together, five fragments were produced of sizes 6.5kb, 4kb, 2kb, 1.4kb, and 0.8kb. Which of the following is the correct restriction map on this molecule
A. A: 7.3kb E 4.8kb B 2kb B 4kb E
B. 4kb E 0.8kb B 6.5kb E 1.4kb B 2kb
C. 6.5kb B 4kb E 2kb E 1.4kb B 0.4kb
D. 6.5kb E 4kb E 2kb B 1.4kb B 0.4kb
E. 4kb B 0.8kb E 6.5kb B 1.4kb E 2kb
Homework Answers
The correct option is B
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A linear DNA molecule was digested with restriction enzyme EcoR
I (E) and three fragments were...
A 10 kb linear DNA molecule was digested with restriction enzyme EcoRI (E) and two fragments...
A 10 kb linear DNA molecule was digested with restriction enzyme EcoRI (E) and two fragments were produced of sizes 6 kb and 4 kb. The restriction enzyme HaeIII (H) also produced two fragments of sizes 9 kb and 1 kb. When the two enzymes HaeIII and EcoRI were used together, three fragments were produced of sizes 1 kb, 3 kb, and 6 kb. A 4 kb long cloned genomic probe from this region hybridized to the 3 kb and...
A linear DNA molecule that is 3250 bp long is digested with restriction enzyme A alone,...
A linear DNA molecule that is 3250 bp long is digested with restriction enzyme A alone, restriction enzyme B alone, or a combination of enzymes A and B to produce the following fragment sizes. Construct a restriction map of the DNA fragment. How many times does enzyme A cut the linear DNA molecule? If you are unable to place both enzymes A and B on the map, show one possible arrangement of enzyme A cognition sites on the DNA molecule.
5. You have a linear DNA fragment and you wish to generate a restriction map using...
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I need help making a restriction digest map for both of these sets of fragments. Please...
I need help making a
restriction digest map for both of these sets of fragments. Please
explain the steps and include drawing.
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Hi I have a problem with number 5, it involves gel analysis results. There are 2 parts, a,b,c. For A Im sure you need to make a graph with distance in (cm) on the vertical axis and log10 bp on the hor...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
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5. You have a linear DNA fragment and you wish to generate a restriction map using Mstl and EcoR1. When the framgent is digested with Mst1 and the DNA is run on a gel you observe a 4kb and a 6kb fragment. When the DNA is digested with EcoR1, 1kb, 4kb and 5kb fragments are generated, A double digest using both enzymes produce 1kb, 2kb, 3kb and 4kb fragments. Explain these results and draw a restirction map consistent with these...
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The figure below shows a restriction map of a segment of a DNA molecule. Eco refers to locations where the restriction endonuclease EcoRI cuts the DNA, and Pst refers to locations where the restriction enzyme Pst cuts the DNA. Potential restriction sites are numbered 1-6. Distances between restriction sites are shown on the bottom scale in base pairs (bp). The thick line represents the part of the molecule that has homology with a probe. Eco Pst Eco Pst Eco Pst...
I need help making a
restriction digest map for both of these sets of fragments. Please
explain the steps and include drawing.
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Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
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