4. How would you clone the Bacillus thuringiensis insecticidal gene into corn plant? Be specific about...
4. How would you clone the Bacillus thuringiensis insecticidal gene into corn plant? Be specific about the procedures and the enzymes used in the process. MICRO 02
Homework Answers
Request Answer!
We need at least 10 more requests to produce the answer.
0 / 10 have requested this problem solution
The more requests, the faster the answer.
Add Answer to:
4. How would you clone the Bacillus thuringiensis
insecticidal gene into corn plant? Be specific about...
You are tasked to clone the human actin gene (blue) from a cDNA clone that you...
You are tasked to clone the human actin gene (blue) from a cDNA clone that you obtained from a collaborator into the cloning vector termed pUC119 using the HindIII restriction enzyme. The 4 blue arrows show the HindIII recognition sequence on the Actin cDNA clone. Plac MCS Hindill Sphl sbil Pst BspMI Accl Hincli Sall Xbal BamHI Smal Xmal Acc651 Kpnl Eco53ki Sacl EcoRI lacz pMB1 ori M13 IG PUC119 3162 bps CDNA Clone Amp a. b. Figure 2: a....
You are interested to know if a commonly used pesticide causes the corn plant to develop...
You are interested to know if a commonly used pesticide causes the corn plant to develop tumors. To test this, you use spray different corn plants with different concentrations of the pesticide and later count the number of tumors that each plant develops. If you were to plot the data, how would you label the two axes on your graph? x axis (horizontal) y axis (vertical) Which of these axes is the independent variable (the X or the Y axis)?...
You have cloned the Pepsi Gene into the vector pKEN. You used two restriction enzymes, BamHI...
You have cloned the Pepsi Gene into the vector pKEN. You used two restriction enzymes, BamHI and EcoRI to force the cloning in a directional way. The plasmid (pKEN) is ~5 kb long and the insert Pepsi 800bp long. After transformation into E. coli you grow up several colonies and isolate the vector. After digestion of the vector it looks like you have three potential clones. You need to sequence the gene to make sure you have the correct clone....
How to make gmo corn? a step by step process how you would achieve this. (which...
How to make gmo corn? a step by step process how you would achieve this. (which vectors, plasmids, DNA, organisms, mode of injecting new DNA).
Question 20 4 pts You have discovered a gene that enables organisms to accumulate gold in...
Question 20 4 pts You have discovered a gene that enables organisms to accumulate gold in their tissues by concentrating trace amounts found in normal soil. You want to transfer this gene into a plant. Order the steps below that would accomplish this goal. 1. Infect the plant with the Agrobacterium strain. 2. Digest the gold gene and a Ti plasmid with appropriate restriction enzymes. 3. Insert the gold gene into the Ti plasmid. 4. Amplify the gold gene with...
16)How can you show experimentally that that day-length signal is perceived by leaves? 17)What specific gene...
16)How can you show experimentally that that day-length signal is perceived by leaves? 17)What specific gene is induced as a result of day length? Wherein the plant this gene is activated? Then how does this gene activate other genes in the SAM? 18)Transition of SAM into inflorescence meristem is regulated by several genes. With known knowledge how do you explain what is happening during SAM to IM transition? 19)Using ABC model explain how the different structures are formed in developing...
As a nurse, how would you reveal a terminal diagnosis to your patient? be specific about...
As a nurse, how would you reveal a terminal diagnosis to your patient? be specific about how you would incorporate insights.
would you please help me with this question? 2. How does site specific recombination allow DNA...
would you please help me with this question?
2. How does site specific recombination allow DNA to b What types of mobile elements can move around the genome? What enzymes catalyze the process? e exchanged between unrelated DNA sequences?
4) RNA interference (RNAi) can be used to silence specific genes in specific tissues. You inject...
4) RNA interference (RNAi) can be used to silence specific genes in specific tissues. You inject various RNAi constructs into Drosophila embryos. in each of the following cases? (3pts) What effect on phenotype would you expect a) One gap gene silenced b) Bicoid silenced c) One Hox gene silenced
ANSWER GENERALLY NOT ABOUT ANY SPECIFIC TOPIC -. How would you explain to a new linear...
ANSWER GENERALLY NOT ABOUT ANY SPECIFIC
TOPIC
-. How would you explain to a new linear algebra student the basic steps to writing a proof.
You are tasked to clone the human actin gene (blue) from a cDNA clone that you obtained from a collaborator into the cloning vector termed pUC119 using the HindIII restriction enzyme. The 4 blue arrows show the HindIII recognition sequence on the Actin cDNA clone. Plac MCS Hindill Sphl sbil Pst BspMI Accl Hincli Sall Xbal BamHI Smal Xmal Acc651 Kpnl Eco53ki Sacl EcoRI lacz pMB1 ori M13 IG PUC119 3162 bps CDNA Clone Amp a. b. Figure 2: a....
You are interested to know if a commonly used pesticide causes the corn plant to develop tumors. To test this, you use spray different corn plants with different concentrations of the pesticide and later count the number of tumors that each plant develops. If you were to plot the data, how would you label the two axes on your graph? x axis (horizontal) y axis (vertical) Which of these axes is the independent variable (the X or the Y axis)?...
Question 20 4 pts You have discovered a gene that enables organisms to accumulate gold in their tissues by concentrating trace amounts found in normal soil. You want to transfer this gene into a plant. Order the steps below that would accomplish this goal. 1. Infect the plant with the Agrobacterium strain. 2. Digest the gold gene and a Ti plasmid with appropriate restriction enzymes. 3. Insert the gold gene into the Ti plasmid. 4. Amplify the gold gene with...
would you please help me with this question?
2. How does site specific recombination allow DNA to b What types of mobile elements can move around the genome? What enzymes catalyze the process? e exchanged between unrelated DNA sequences?
4) RNA interference (RNAi) can be used to silence specific genes in specific tissues. You inject various RNAi constructs into Drosophila embryos. in each of the following cases? (3pts) What effect on phenotype would you expect a) One gap gene silenced b) Bicoid silenced c) One Hox gene silenced
ANSWER GENERALLY NOT ABOUT ANY SPECIFIC
TOPIC
-. How would you explain to a new linear algebra student the basic steps to writing a proof.
Most questions answered within 3 hours.
-
Where is the error in this code sequence?
String s1 = "Hello";
String s2 = "ello";...
asked 10 months ago -
Financial data for Joel de Paris, Inc., for last year
follow:
Joel de Paris, Inc.
Balance...
asked 10 months ago -
Consider this reaction:
Al2(SO4)3 (aq)+ BaCl3
(aq) Al2Cl6 (aq)- +
3BaSO4(s) . What is the...
asked 10 months ago -
Suppose that Savneet is considering increasing her
recent random sample from 20 car rentals to 40...
asked 10 months ago -
Trucks arrive at an unloading terminal at an average rate of 120
per hour.
Trucks arrive...
asked 10 months ago -
Why are methanol and ethanol completely soluble in water while
octanol is not very little soluble....
asked 10 months ago -
A facilities manager at a university reads in a research report
that the mean amount of...
asked 10 months ago -
When the CuSO4 is rehydrated by adding water to the anhydrous
compound, is this an endothermic...
asked 10 months ago -
A ray of sunlight is passing from diamond into crown glass; the
angle of incidence is...
asked 10 months ago -
A block of mass 0.249 kg is placed on top of a light, vertical
spring of...
asked 10 months ago -
how do the kidneys compensate in the presences of acidosis
a) trigger hyperventilate
b) reserve acid...
asked 10 months ago -
Question 501 pts
The rental rate of capital to the firm increases. Which of the
following...
asked 10 months ago