How to make gmo corn? a step by step process how you would achieve this. (which...
How to make gmo corn? a step by step process how you would achieve this. (which vectors, plasmids, DNA, organisms, mode of injecting new DNA).
Homework Answers
Hi,
The GMO corn is produced by Monsanto company to develop insect resistance in corn. The idea was to insert the Cry1AB gene from bacteria Bacillus thuringensis into corn, which will make it resistant to insect infestation.
The gene of interest is PCR amplified and purified from the source bacteria. The isolated gene is restriction digested with desired enzymes such as BamH1 or EcoR1. It is now ready to insert into a plasmid. A suitable plant plasmid such as pCAMBIA is used to carry the gene into the corn via agrobacterium. The gene is ligated with the plamsid and multiplied inside another bacteria Agrobacterium. Here the gene is amplified along with the plasmid and multiple copies are made. These are now extracted and coated onto micro tungsten beads. These beads are bombarded under pressure onto the vegetative tissue like leaf of corn. The bombarded tissue is used as Ex plant for tissue culture. A proper callus is allowed to form and slowly new shoots emerges. This is now confirmed for the presence of gene of interest. If it is positive, then we have created a GMO corn.
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