What is the expected methylation status of a housekeeping gene versus a transposon? Give a brief...
What is the expected methylation status of a housekeeping gene versus a transposon? Give a brief explanation.
Homework Answers
Epigenetic regulation is critical for proper development and cellular differentiation in both plants and animals. Epigenetic modifications do not alter the DNA sequence but affect the gene expression by Histone modifications and DNA methylation. DNA methylation is a very stable and reversible modification. It is highly correlated with the gene specific transcriptional status. DNA methylation occurs at the C-5 position of cytosine residues of the CpG dinucleotide sequence that generally results in repression of transcriptional activity. DNA methylation is involved several key processes such as aging, carcinogenesis, X-chromosome inactivation and repression of transposable elements.
Expression of housekeeping genes is essential of the proper cellular metabolism. So, methylation does not normally occur at the house keeping gene loci. Transcription of transposons has to be repressed. So, their genomic locations are highly methylated.
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What is the expected methylation status of a housekeeping gene
versus a transposon? Give a brief...
2. Give 2-3 examples of gene regulation in eukaryotes. Define what they are and how they...
2. Give 2-3 examples of gene regulation in eukaryotes. Define what they are and how they specifically regulate gene expression 3. What is histone acetyltransferase enzyme? How does it regulate gene expression? Explain your reasons 4. What is chromatin remodeling in general? 5. What is DNA methylation? What does methylation mean in this case? How does it intervene gene expression? Explain your reason
Explain how DNA methylation could be used to regulate gene expression in a tissue-specific way. When...
Explain how DNA methylation could be used to regulate gene expression in a tissue-specific way. When and where would de novo methylation occur, and when would demethylaiton occur? What would occur in the cells that give rise to eggs and sperm (germ-line cells)
Explain how DNA methylation could be used to regulate gene expression in a tissue-specific way. When...
Explain how DNA methylation could be used to regulate gene expression in a tissue-specific way. When and where would de novo methylation occur, and when would demethylaiton occur? What would occur in the cells that give rise to eggs and sperm (germ-line cells)
5. What is the connection between DNA methylation, histone deacetylation, and gene regulation in eukaryotes?
5. What is the connection between DNA methylation, histone deacetylation, and gene regulation in eukaryotes?
What three methodologies were used to assess gene expression? Give a brief description of each methodology (the...
What three methodologies were used to assess gene expression? Give a brief description of each methodology (the principle of the methodology, not a summary of the actual experiment performed). What is/are the primary advantage(s) of each method? (15 marks)
What three aspects of a gene are described by GO terms? Give the name and a...
What three aspects of a gene are described by GO terms? Give the name and a brief description of each in your own words.
What would be the expected outcome of a mutation in E.coli that prevented methylation of adenine...
What would be the expected outcome of a mutation in E.coli that prevented methylation of adenine in the sequence 5'-GATC-3'? A) loss of epigenetic markers B) Randomization of mismatch repair C) increased mutation rate D) B and C E) All of these
1. What is the definition of prevention? 2. Give 4 examples of prevention and a brief...
1. What is the definition of prevention? 2. Give 4 examples of prevention and a brief explanation of each on. 3. What is the definition of treatment? 4. Give 4 examples of a treatment and a brief description of each one.
) What are the initial conditions? (No work to show, but please give a brief explanation...
) What are the initial conditions? (No work to show, but please give a brief explanation for why they are what they are.)
Explain the difference between translational, post-translational, transcriptional, and post-transcriptional gene regulation. Give brief examples of each.
Explain the difference between translational, post-translational, transcriptional, and post-transcriptional gene regulation. Give brief examples of each.
2. Give 2-3 examples of gene regulation in eukaryotes. Define what they are and how they specifically regulate gene expression 3. What is histone acetyltransferase enzyme? How does it regulate gene expression? Explain your reasons 4. What is chromatin remodeling in general? 5. What is DNA methylation? What does methylation mean in this case? How does it intervene gene expression? Explain your reason
5. What is the connection between DNA methylation, histone deacetylation, and gene regulation in eukaryotes?
1. What is the definition of prevention? 2. Give 4 examples of prevention and a brief explanation of each on. 3. What is the definition of treatment? 4. Give 4 examples of a treatment and a brief description of each one.
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