Homework Answers
Add Answer to:
biochemistry calculate enzyme activity
enzyme activily Calucabion 0.0157 min/ mH 0.2mL unit should be
Help! Calculate the concentration of lysozyme activity if 150uL of enzyme is added to 850uL of...
Help! Calculate the concentration of lysozyme activity if 150uL of enzyme is added to 850uL of substrate to yield a) -0.0435abs/min at A450? b) 0.000abs/min at A450? The blank used was water...abs/min=0.000. df is 20
You have a 25 ml enzyme sample that has a total of 100 micro-moles/min of activity...
You have a 25 ml enzyme sample that has a total of 100 micro-moles/min of activity If you were to assay the enzyme, how much of it would you need in your assay in order to get an absorbance of 0.50 in a 30-minute assay. The product E=13,800M-1cm-1 and you are using a standard 1mL cuvette Beer-lambert law: A=CIE I=1
You have a 25 ml enzyme sample that has a total of 100 micro-moles/min of activity If you were to...
You have a 25 ml enzyme sample that has a total of 100 micro-moles/min of activity If you were to assay the enzyme, how much of it would you need in your assay in order to get an absorbance of 0.50 in a 30-minute assay. The product E=13,800M-1cm-1 and you are using a standard 1mL cuvette Beer-lambert law: A=CIE I=1
[Alcohol] mMAD Activity AD Activity AD Activity mM/min Agent A Agent B mM/minmM/min 15.0 2.5 5.0 7.5 45.0 20.0 0.5...
[Alcohol] mMAD Activity AD Activity AD Activity mM/min Agent A Agent B mM/minmM/min 15.0 2.5 5.0 7.5 45.0 20.0 0.5 63.0 10.0 32.0 71.0 2.0 11.7 48.0 4.0 79.0 13.4 66.0 8.0 14.3 83.0 84.0 32.0 90.0 15.5 90.0 1. Using the data provided draw a Michaelis-Menten and Lineweaver-Burk Plot for each of the enzyme activities? When you produce the 2 graphs make sure you have plotted 3 curves for the Michaelis-Menten plot on one graph and the three lines...
If your specific activity of your enzyme is 20 micromoles of product/(min*mg protein), how many micromols...
If your specific activity of your enzyme is 20 micromoles of product/(min*mg protein), how many micromols of product can be attained in 2 minutes given 2 mg of protein? a. 5 mol b. 20 mol c. 80 mol d. cannot be determined from the data provided in the problem
1. The turnover number for an enzyme is known to be 5000 min. From the following set of data, calculate the Km, Vma...
1. The turnover number for an enzyme is known to be 5000 min. From the following set of data, calculate the Km, Vmax and the amount of enzyme present in this experiment. Use excel to obtain the lineweaver burk plot. Substrate concentration (MM) 1 Initial velocity (umol/min) 167 250 334 376 498 499 100 1,000
A crude cell extract containing 2 g.L -1 of protein was assayed for enzyme activity using...
A crude cell extract containing 2 g.L -1 of protein was assayed for enzyme activity using an excess of substrate. It was found that 5 ml of the solution converted 25 µmoles of substrate to product over a period of 2 minutes. (i) Calculate the specific activity of the enzyme. The crude extract was subjected to anion exchange chromatography followed by gel filtration. After anion exchange the specific activity had risen to 226 µmoles.min-1 .mg-1 , and after gel filtration...
Calculate the specific activity for each sample using the formula: 1 unit (specific activity) = 1000...
Calculate the specific activity for each sample using the
formula:
1 unit (specific activity) = 1000 x (A420– (1.75 x
A550) / (T x V x A600)
where:
A420= absorbance of the yellow ONP
A550= cell debris light scattering
T = reaction time in minutes
V = volume of culture assayed (ml)
A600= cell density
Sample
Calculation
Activity
1
2
3
4
5
6
7
8
9
10
β galactosidase Activity (10 points) RESULS Ex. 1 1 a: Acoo |...
The specific activity of a pure enzyme is 6000 μmol substrate transformed.min-1.(mg protein)-1 and its molecular...
The specific activity of a pure enzyme is 6000 μmol substrate transformed.min-1.(mg protein)-1 and its molecular weight is 10,000 Da. Calculate the turnover number of the enzyme in units of μmol substrate transformed.(μmol enzyme)-1.sec-1]? Show all workings including units. Note: A calculator should not be required to answer this question.
17. How should the specific activity of an enzyme preparation change during its purification? A. Increase...
17. How should the specific activity of an enzyme preparation change during its purification? A. Increase B. Decrease C. Remain unchanged D. Unknown; it must be determined for each enzyme individually E. Enzymes cannot be purified 18 18. Which amino acid is being titrated in the figure to the right? A. D B.G C. H D. K E. R NOH The next question concerns the SDS-PAGE figure below. Lane 1 contains an unknown sample. Lane M contains a mixture of...
[Alcohol] mMAD Activity AD Activity AD Activity mM/min Agent A Agent B mM/minmM/min 15.0 2.5 5.0 7.5 45.0 20.0 0.5 63.0 10.0 32.0 71.0 2.0 11.7 48.0 4.0 79.0 13.4 66.0 8.0 14.3 83.0 84.0 32.0 90.0 15.5 90.0 1. Using the data provided draw a Michaelis-Menten and Lineweaver-Burk Plot for each of the enzyme activities? When you produce the 2 graphs make sure you have plotted 3 curves for the Michaelis-Menten plot on one graph and the three lines...
1. The turnover number for an enzyme is known to be 5000 min. From the following set of data, calculate the Km, Vmax and the amount of enzyme present in this experiment. Use excel to obtain the lineweaver burk plot. Substrate concentration (MM) 1 Initial velocity (umol/min) 167 250 334 376 498 499 100 1,000
Calculate the specific activity for each sample using the
formula:
1 unit (specific activity) = 1000 x (A420– (1.75 x
A550) / (T x V x A600)
where:
A420= absorbance of the yellow ONP
A550= cell debris light scattering
T = reaction time in minutes
V = volume of culture assayed (ml)
A600= cell density
Sample
Calculation
Activity
1
2
3
4
5
6
7
8
9
10
β galactosidase Activity (10 points) RESULS Ex. 1 1 a: Acoo |...
17. How should the specific activity of an enzyme preparation change during its purification? A. Increase B. Decrease C. Remain unchanged D. Unknown; it must be determined for each enzyme individually E. Enzymes cannot be purified 18 18. Which amino acid is being titrated in the figure to the right? A. D B.G C. H D. K E. R NOH The next question concerns the SDS-PAGE figure below. Lane 1 contains an unknown sample. Lane M contains a mixture of...
Most questions answered within 3 hours.
-
Where is the error in this code sequence?
String s1 = "Hello";
String s2 = "ello";...
asked 10 months ago -
Financial data for Joel de Paris, Inc., for last year
follow:
Joel de Paris, Inc.
Balance...
asked 10 months ago -
Consider this reaction:
Al2(SO4)3 (aq)+ BaCl3
(aq) Al2Cl6 (aq)- +
3BaSO4(s) . What is the...
asked 10 months ago -
Suppose that Savneet is considering increasing her
recent random sample from 20 car rentals to 40...
asked 10 months ago -
Trucks arrive at an unloading terminal at an average rate of 120
per hour.
Trucks arrive...
asked 10 months ago -
Why are methanol and ethanol completely soluble in water while
octanol is not very little soluble....
asked 10 months ago -
A facilities manager at a university reads in a research report
that the mean amount of...
asked 10 months ago -
When the CuSO4 is rehydrated by adding water to the anhydrous
compound, is this an endothermic...
asked 10 months ago -
A ray of sunlight is passing from diamond into crown glass; the
angle of incidence is...
asked 10 months ago -
A block of mass 0.249 kg is placed on top of a light, vertical
spring of...
asked 10 months ago -
how do the kidneys compensate in the presences of acidosis
a) trigger hyperventilate
b) reserve acid...
asked 10 months ago -
Question 501 pts
The rental rate of capital to the firm increases. Which of the
following...
asked 10 months ago