8. You want to design a plasmid vector that would enable researchers to have their favorite...
8. You want to design a plasmid vector that would enable researchers to have their favorite protein(s) secreted by E. coli, even though that protein is not normally a secreted protein. Which of the secretion systems would you choose and why? Describe what this plasmid vector would entail.
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8. You want to design a plasmid vector that would enable
researchers to have their favorite...
2. You are trying to design a protein that will be expressed in Escherichia coli and...
2. You are trying to design a protein that will be expressed
in Escherichia coli and secreted outside of the cell for
purification and use as a pharmaceutical. E. coli is a
gram-negative cell and the protein folds after it has exited the
cell. Which secretion system would work best for this project?
Support your answer with evidence based on the properties of E.
coli, the protein, and the secretion system. (20 pts)
2. You are trying to design a...
You are trying to design a protein that will be expressed in Escherichia coli and secreted...
You are trying to design a protein that will be expressed in Escherichia coli and secreted outside of the cell for purification and use as a pharmaceutical. E. coli is a gram-negative cell and the protein folds after it has exited the cell. Which secretion system would work best for this project? Support your answer with evidence based on the properties of E. coli, the protein, and the secretion system
You are trying to design a protein that will be expressed in Escherichia coli and secreted...
You are trying to design a protein that will be expressed in Escherichia coli and secreted outside of the cell for purification and use as a pharmaceutical. E,coli is a gram-negative cell and the protein folds after it has existed the cell. Which secretion system would work best for this project? Support your answer with evidence based on the properties of E.coli, the protein , and the secretion system.
A fellow researcher is constructing a plasmid vector they are hoping to express in e. coli....
A fellow researcher is constructing a plasmid vector they are hoping to express in e. coli. As they are designing their experimental protocols they are concerned that they will not be able to identify colonies which were successfully transformed, with the correct plasmid/insertion construct. Provide a suggested mechanism or method to address their concerns. Specifically answer the following two questions and provide details on the biological mechanism underpinning the method. a. Describe a mechanism to determine if the bacterial have...
NOTE: this will require that you remember some stuff from bacterial genetics. You want to research...
NOTE: this will require that you remember some stuff from
bacterial genetics.
You want to research the eukaryotic protein BabyFeet, which
smells like peppermint. To make cDNA you need...
a) You prepare plates to grow your bacteria on. Your competent
E. coli are resistant to penicillin, cephalexin, and susceptible to
ampicillin. Which antibiotic will you put in your media?
b) Next, you prepare your cloning vector, which contains the
genes Ampr, cepr, and penr all downstream of the promoter. The...
8. (There are three main classes of plasmid vector used in S. cerevisiae: YEp, YCp and...
8. (There are three main classes of plasmid vector used in S. cerevisiae: YEp, YCp and YIp plasmids. a) Which of these would you use in order to find the true complementing gene of a mutant phenotype and why? b) Which of these would you use in a genetic (chromosomal) mapping study and why?
You have cloned the Pepsi Gene into the vector pKEN. You used two restriction enzymes, BamHI...
You have cloned the Pepsi Gene into the vector pKEN. You used two restriction enzymes, BamHI and EcoRI to force the cloning in a directional way. The plasmid (pKEN) is ~5 kb long and the insert Pepsi 800bp long. After transformation into E. coli you grow up several colonies and isolate the vector. After digestion of the vector it looks like you have three potential clones. You need to sequence the gene to make sure you have the correct clone....
A plasmid used as a cloning vector in E. coli must have… Does sequence similarity between...
A plasmid used as a cloning vector in E. coli must have… Does sequence similarity between genes play an important role in assigning gene function? Successful insertion of a DNA fragment into the multi-cloning region (restriction sites) of a recombinant plasmid is detected by what changes? Understand the concept of (restriction enzyme produced) DNA fragment separation by gel electrophoresis. In addition to restriction enzymes, which enzyme(s) are required to insert a fragment of DNA into a cloning vector? What is...
Suppose you have a dna fragement you would like to insert into the psap plasmid. the...
Suppose you have a dna fragement you would like to insert into the psap plasmid. the fragement has pstl and ecorl restriction endonuclease sites near the 5' end and hindlll and smal restriction endonuclease sites near the 3' end. choose the best restriction endonuclease(s) to digest the both the dna fragment and psap.
Consider a job you hold now or have held recently. Would you want this job to...
Consider a job you hold now or have held recently. Would you want this job to be redesigned to place more emphasis on efficiency, motivation, ergonomics, or mental processing? Explain why you choose the specific factor. What changes would you want and why?
2. You are trying to design a protein that will be expressed
in Escherichia coli and secreted outside of the cell for
purification and use as a pharmaceutical. E. coli is a
gram-negative cell and the protein folds after it has exited the
cell. Which secretion system would work best for this project?
Support your answer with evidence based on the properties of E.
coli, the protein, and the secretion system. (20 pts)
2. You are trying to design a...
NOTE: this will require that you remember some stuff from
bacterial genetics.
You want to research the eukaryotic protein BabyFeet, which
smells like peppermint. To make cDNA you need...
a) You prepare plates to grow your bacteria on. Your competent
E. coli are resistant to penicillin, cephalexin, and susceptible to
ampicillin. Which antibiotic will you put in your media?
b) Next, you prepare your cloning vector, which contains the
genes Ampr, cepr, and penr all downstream of the promoter. The...
Suppose you have a dna fragement you would like to insert into the psap plasmid. the fragement has pstl and ecorl restriction endonuclease sites near the 5' end and hindlll and smal restriction endonuclease sites near the 3' end. choose the best restriction endonuclease(s) to digest the both the dna fragment and psap.
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