An investigator would be able to distinguish a solution containing single-stranded DNA from one containing double-stranded...
An investigator would be able to distinguish a solution containing single-stranded DNA from one containing double-stranded DNA by
a. Cooling the solutions to 16°C and measuring the absorption of light at 260 nm.
b. Measuring the absorption at 280 nm.
c. Monitoring the change in absorption of light at 260 nm while elevating the temperature.
Homework Answers
(C) Monitoring the change in absorption of light at 260 nm while elevating the temperature.
Because, ssDNA can be differentiated from dsDNA as ssDNA has a greater level of absorption at 260nm when the temperature is elevated subsequently because of hyperchromic shift as the bases are not stacked as in dsDNA.
The results can thereafter be confirmed by running an electrophoresis experiment where ssDNA can be differentiated from dsDNA as ssDNA will run more rapidly on the gel when compared to the other.
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S
G2...
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