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1) Explain how we can use changes in PH to identify organisms that ferment certain sugars.

1) Explain how we can use changes in PH to identify organisms that ferment certain sugars.

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Answer #1

When microorganisms ferment carbohydrates an acid or acid gas are produced. Commonly used substrates are glucose, lactose, sucrose etc. Depending upon the organism involved and the substrates being fermented, the end product can be lactic acid, formic acid, acetic acid, butyric acid, buthyl alcohol, carbondioxide, acetone and hydrogen.

If acid is formed after fermentation it lowers the PH of the medium, which can be detected by change in color of PH indicator.

Color change only occur when sufficient amount of acid is produced, as bacteria may utilize the peptone producing alkaline by product.

Commonly used PH indicator in carbohydrates fermentation is phenol red. Other PH indicator used are bromocresol, bromothymol.

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