12. Given a series of 4 dilutions, each 1/5, what is the concentration in tube No....
12. Given a series of 4 dilutions, each 1/5, what is the concentration in tube No. 3 if the original concentration was 100 mg/dL?
13. A 1/2, fourfold serial dilution was performed. The original concentration of the analyte was 1200 μg/dL. What is the concentration of the analyte in the final tube?
7. Calculate the true analyte value given the following information: Analyte diluted using 10 μL into 90 μL diluent; Diluted analyte value = 85 mg/dL.
Homework Answers
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12. Given a series of 4 dilutions, each 1/5, what is the
concentration in tube No....
13. A 1/2, fourfold serial dilution was performed. The original concentration of the analyte was 1200...
13. A 1/2, fourfold serial dilution was performed. The original concentration of the analyte was 1200 μg/dL. What is the concentration of the analyte in the final tube? 7. Calculate the true analyte value given the following information: Analyte diluted using 10 μL into 90 μL diluent; Diluted analyte value = 85 mg/dL.
o Calculate the following sample concentrations based upon given dilutions. Final (Diluted) Concentration 1st Dilution 2nd...
o Calculate the following sample concentrations based upon given dilutions. Final (Diluted) Concentration 1st Dilution 2nd Dilution Concentration Original 1:5 1:4 1:15 1:3 1:3 3 (none) 1:2 1:5 (none) 1:5 167 mg/dL b. 139 mg/dL 15 mg/dL d. 557 mg/dL 23 mg/dL Page 2 of 3
dilutions dilutions What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L...
dilutions
dilutions
What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L of serum is diluted to 2.0 mL 50 uL of whole blood is pipetted into 0.95 mL of diluent 500 mL of liquid K is added to 4.5 L of water 7. If 10 uL of urine is added to 90 uL of water, and the resulting solution is brought to a final volume of 0.50 mL with water, what is the final dilution...
1) if 5 parts sample is added to 10 parts diluents, what is the dilution that...
1) if 5 parts sample is added to 10 parts diluents, what is the dilution that was just made? 2. What is the ratio if 5 parts sample volume is added to 10 parts diluents volume? 3. In a 1 to 3 dilution, what is the dilution FACTOR? 4. In a 1 to 10 dilution, what is the dilution FACTOR? 5. What is the dilution FACTOR if 40 mcL of sample added to 260 mcL of diluent? 6. What is...
Also— what is the dilution factor in tube 2? please show the steps to the answers!...
Also— what is the dilution factor in tube 2?
please show the steps to the answers!
Practice Problems Solve the following practice problems to further master the material. Answers and explanations to some problems can be found at the end of the book. Calculate the dilution factors for the following problems: 1.5 mL sample added to 95 mL diluent 2. 20 mL sample added to 180 mL diluent 3. 40 mL sample added to 200 mL diluent 4. 2 mL...
please do 5, 6, 7 and also please tell me the dilution factor of tube two...
please do 5, 6, 7
and also please tell me the dilution factor of tube two (that
is number 8 on a different page)
Practice Problems solve the following practice problems to further master the material. Answers and explanations to some problems can be found at the end of the book. Calculate the dilution factors for the following problems: 1.5 mL sample added to 95 mL diluent 2. 20 mL sample added to 180 mL diluent 3. 40 mL sample...
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 3...
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2.00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of 100.00 mL. Calculate...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to make a set of standards with the concentrations shown below. You need a minimum of 500 l of each standard left after you have finished all your dilutions. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below include the final dilution of the standard as compared to the original stock concentration and the final...
The standard concentration of the antibiotic Vancomycin is 6.20 mg/dl. It is diluted 1:10. Then 1.0...
The standard concentration of the antibiotic Vancomycin is 6.20 mg/dl. It is diluted 1:10. Then 1.0 ml of the first dilution is added to 4.0 ml of diluent? What is the final concentration of the antibiotic in this solution?
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
o Calculate the following sample concentrations based upon given dilutions. Final (Diluted) Concentration 1st Dilution 2nd Dilution Concentration Original 1:5 1:4 1:15 1:3 1:3 3 (none) 1:2 1:5 (none) 1:5 167 mg/dL b. 139 mg/dL 15 mg/dL d. 557 mg/dL 23 mg/dL Page 2 of 3
dilutions
dilutions
What are the resulting dilutions? Notice units of measurement. a) b) c) 500 L of serum is diluted to 2.0 mL 50 uL of whole blood is pipetted into 0.95 mL of diluent 500 mL of liquid K is added to 4.5 L of water 7. If 10 uL of urine is added to 90 uL of water, and the resulting solution is brought to a final volume of 0.50 mL with water, what is the final dilution...
Also— what is the dilution factor in tube 2?
please show the steps to the answers!
Practice Problems Solve the following practice problems to further master the material. Answers and explanations to some problems can be found at the end of the book. Calculate the dilution factors for the following problems: 1.5 mL sample added to 95 mL diluent 2. 20 mL sample added to 180 mL diluent 3. 40 mL sample added to 200 mL diluent 4. 2 mL...
please do 5, 6, 7
and also please tell me the dilution factor of tube two (that
is number 8 on a different page)
Practice Problems solve the following practice problems to further master the material. Answers and explanations to some problems can be found at the end of the book. Calculate the dilution factors for the following problems: 1.5 mL sample added to 95 mL diluent 2. 20 mL sample added to 180 mL diluent 3. 40 mL sample...
1. Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2.00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of 100.00 mL. Calculate...
You only have 150 pl stock solution that contains 100 mgml of protein. You want to make a set of standards with the concentrations shown below. You need a minimum of 500 l of each standard left after you have finished all your dilutions. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below include the final dilution of the standard as compared to the original stock concentration and the final...
2) You are given a bacterial culture with the following serial dilutions: 1 ml 0.1 ml 1 ml Original culture 9 ml media 9.9 ml media 9 ml media B a) what is the final dilution in tube C? (1 pt) b) if you plated 100 ul of the bacterial dilution in tube B, incubated and counted 114 colonies the next day, what would the CFU/ml be of the original bacterial culture? (show work and units- 2 pts)
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