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why is it neccessary to have zero a 0 sample (no protein) as part of a...

why is it neccessary to have zero a 0 sample (no protein) as part of a standard curve?

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Answer: The necessity to have zero a sample or setting blank (no protein ) as a part of standard curve is to zero the background absorbance caused by the reagent. After setting blank or zero the (no protein) sample will give the correct concentration of protein present in the different concentrations of the reagent or solution.

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