You buy a protein standard at a concentration of 4mg/200ml. How would you make 1 ml...
You buy a protein standard at a concentration of 4mg/200ml. How would you make 1 ml of a 10ug/ml, working solution?
Homework Answers
With the given concentration of protein 4mg/200ml implies 200ml contains 4mg of protein.
The working concentration is given 10ug/ml which implies 1ml should contain 10ug of protein.
By calculation using unitary method 10ug of protein will require only 50 ul of sample. Thus, if 50 ul of sample is diluted with 950 ul if water it will give the desired working concentration.
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