Question

I was instructed to use the standard curve to determine the protein content of each of the 5 “unknown” samples and record each value in a table. record the protein content value you determined from your standard curve how is this calculated on a graph?

Answer 1

while plotting standard curve of protein content, on X axis Protein content is plotted, it can be protein amount or protein concentration which you have taken while performing the assay. On Y axis, values of Absorbance at a particular wavelength ( depending on the assay at which wavelength Absorbance is recorded) is plotted, which is obtained during the experiment.

Using these two parameters, standard curve graph of protein is obtained, slope obtained should be a straight line.

this is an example of how standard curve should be plotted. Now extrapolating the values of Absorbance of unknown protein samples, perpendicular to the slope, protein content of protein can be calculated. Another way of calculating is in Excel sheet where graph is plotted, using the equation y = mx+c, we can calculate the value of slope. Then this value of slope is used, along with the Absorbance of unknown protein sample to calculate protein content.