5. A digest was performed on a plasmid using two different enzymes, BopI and ZapII. The results...
5. A digest was performed on a plasmid using two different enzymes, BopI and ZapII. The results are given below.
|
BopI |
|
8.0 |
|
ZapII |
|
2.0 kb |
|
2.5 kb |
|
3.5 kb |
|
BopI + ZapII |
|
1.0 kb |
|
1.5 kb |
|
2.0 kb |
|
3.5 kb |
In the space below please draw the plasmid map showing the where the enzymes cut and the sizes of the fragments and total size of the plasmid.
Homework Answers
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5. A digest was performed on a plasmid
using two different enzymes, BopI and ZapII. The results...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...
34. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with...
34. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes Smal, Bglil and Aval. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb Smal 5.9 4.3 BgII 8.2 2.0 Aval 5.3 4.9 Smal+ BglII 5.4 2.8 0.5 Smal+ Aval 3.3 2.6 2.3 2.0 Smal+-Bg!!! 5.3 2.1 2.0 0.8 (a) Draw an agarose gel with restriction fragments from the data...
please help me with these questions Lab 8 Extension Activity: Plasmid Mapping and Restriction Enzymes Mapping...
please help me with these questions
Lab 8 Extension Activity: Plasmid Mapping and Restriction Enzymes Mapping the Plasmid The first step in mapping a plasmid is to determine how many times a restriction site is found on that plasmid. Examine the results for plasmid 55 as an example. The data given in the following table are for the double digest using EcoRI and Pstl. Also, giving are the data for single digests by the individual enzymes. The numbers in the...
The table below presents the results of different digests of a plasmid. All sizes are in...
The table below presents the results of different digests of a plasmid. All sizes are in base pairs. Digest Size of Fragments HindIII 4.00 kb BamHI 2.35 kb, 1.65 kb EcoRI 4.00 kb, 2.50 kb,1.50 kb HindIII + BamHI 1.65 kb, 1.50 kb, 0.85 kb HindIII + EcoRI 3.50 kb, 3.00 kb, 2.50kb, 1.00 kb, 0.50 kb BamHI + EcoRI 2.00kb, 1.85kb, 1.65kb, 1.5kb, 0.35kb, 0.50 kb One of the enzymes used only partially digested the DNA. Which enzyme is...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
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Unanswered Question 6 0/1 pts A restriction digest is performed on a plasmid. The table below shows the sizes of the resulting DNA fragments. 3 enzyme(s) present in the DNA size fragments digest resulting Pstl 10,000 base pairs (bp) Sspl 7,000 bp, 3,000 bp double digest, Estland 4,000 bp, 3,000 bp Sspl Explain the double digest results, what happened to create pieces of these sizes? Your Answer: 4/4 pts Question 7 List the 5 components needed to perform a PCR...
A plasmid that is 3931 base pairs (bp) is digested with two restriction enzymes Nae I...
A plasmid that is 3931 base pairs (bp) is digested with two restriction enzymes Nae I and Nar I. The Nae I digestion produces two fragments. One is 3158 (bp) and the second is 773 bp. The NarI digestion produces two fragments. One is 3181 bp and the second is 750 bp. When the plasmid is digested with both enzymes four fragments are produced. They are 2656 bp, 525 bp, 502 bp, and 248 bp. Draw a circular map of...
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please select answer from options given in [] and are bold, there are 3 parts 1. An 9 kb circular plasmid is cut with the EcoRI restriction enzyme, and the reaction products are run on a DNA gel and stained with ethidium bromide. Bands of 1.0, 3.0 and 5.0 kb are seen. 2. When the same plasmid is cut with both the EcoRI and BamHI restriction enzymes (a double digest), bands of 1.0, 1.5, 3.0, and 3.5 kb are seen....
Hi I have a problem with number 5, it involves gel analysis results. There are 2 parts, a,b,c. For A Im sure you need to make a graph with distance in (cm) on the vertical axis and log10 bp on the hor...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
can someone explain throughly on how to find a-c??? thanks!!! The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel be...
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someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...
34. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes Smal, Bglil and Aval. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb Smal 5.9 4.3 BgII 8.2 2.0 Aval 5.3 4.9 Smal+ BglII 5.4 2.8 0.5 Smal+ Aval 3.3 2.6 2.3 2.0 Smal+-Bg!!! 5.3 2.1 2.0 0.8 (a) Draw an agarose gel with restriction fragments from the data...
please help me with these questions
Lab 8 Extension Activity: Plasmid Mapping and Restriction Enzymes Mapping the Plasmid The first step in mapping a plasmid is to determine how many times a restriction site is found on that plasmid. Examine the results for plasmid 55 as an example. The data given in the following table are for the double digest using EcoRI and Pstl. Also, giving are the data for single digests by the individual enzymes. The numbers in the...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
Unanswered Question 6 0/1 pts A restriction digest is performed on a plasmid. The table below shows the sizes of the resulting DNA fragments. 3 enzyme(s) present in the DNA size fragments digest resulting Pstl 10,000 base pairs (bp) Sspl 7,000 bp, 3,000 bp double digest, Estland 4,000 bp, 3,000 bp Sspl Explain the double digest results, what happened to create pieces of these sizes? Your Answer: 4/4 pts Question 7 List the 5 components needed to perform a PCR...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
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