Question

Which of the following is NOT a reason that each PCR Primer requires a different PCR settings?

-because the complimentary strands of the DNA may be composed of more G-C pairs which would require less energy to denature than a strand with more A-T pairs due to the amounts of hydrogen bonds to break in each.

       
-because the PCR products are different lengths which may require different amounts of time to denature, elongate , and extend the PCR product.

       
-because the primers vary in sequence which may require different times/temperatures to anneal to its compliment on the template DNA.

       
-because the complimentary strands of the DNA may be composed of more G-C pairs which would require more energy to denature than a strand with more A-T pairs due to the amounts of hydrogen bonds to break in each.

       
-because the primers are all different lengths which may require a variety of time/temperatures to anneal to its compliment on the template DNA.

Answer 1

Answer:

because the complementary strands of the DNA may be composed of more G-C pairs which would require less energy to denature than a strand with more A-T pairs due to the amounts of hydrogen bonds to break in each.

Explanation:

The number of hydrogen bonds between guanine and cytosine is three and between adenine and thymine is two. The energy needed to break the three hydrogen bonds will be more than that for the two hydrogen bonds. Thus, the primer needs different PCR settings, as the complementary strands with more G-C (guanine-cytosine) pairs would require more energy to denature.