Question

How do you expect the K_M determined with a substrate that is preferred by the enzyme to differ from the K_M determined with a general enzyme substrate?

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Answer 1

We all know that enzymes are substrate specific so the substrate that is preferred by the enzyme will catalyzed faster than the general substrate.

K_m is the michaelis menten constant can be defined as the substrate concentration required for the enzyme to attain half maximum velocity of the reaction or 1/2 V_max.

So it is understandable and acceptable that the substrate that is preferred by the enzyme will react faster and will attain V_max early than the other substrate. As V_max is short so value of K_m also will be less as it is directly proportional to V_max. So the value of K_m of the substrate that is preferred by the enzyme will be less than the K_m determined with a general enzyme substrate.

Find the attached image file for graphical explanation